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Abstract

A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12, was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90 % of added propanil (100 mg l) within 3 days of incubation. Growth occurred in the presence of 0–4.5 % (w/v) NaCl (optimum 0.5 %), at 10–40 °C (optimum 28 °C) and at pH 5.5–10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (Cω6 and/or Cω7). The genomic DNA G+C content of strain Y12 was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12 was a member of the genus , as it showed highest sequence similarities to DCA-1 (99.1 %) and <96.0 % similarities with other species of the genus . Strain Y12 showed low DNA–DNA relatedness values with DCA-1. Based on phenotypic, genotypic and phylogenetic properties, strain Y12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y12 ( = CCTCC AB 2010218  = KCTC 23298). An emended description of the genus is also presented.

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2012-03-01
2024-03-28
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References

  1. Anil Kumar P., Srinivas T. N. R., Sasikala Ch., Ramana Ch. V. 2007; Rhodobacter changlensis sp. nov., a psychrotolerant, phototrophic alphaproteobacterium from the Himalayas of India. Int J Syst Evol Microbiol 57:2568–2571 [View Article][PubMed]
    [Google Scholar]
  2. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230[PubMed] [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  8. Gregersen T. 1978; Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5:123–127 [View Article]
    [Google Scholar]
  9. Guo P., Wang B. Z., Hang B. J., Li L., Ali S. W., He J., Li S. 2009; Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase. Int Biodeterior Biodegradation 63:1107–1112 [View Article]
    [Google Scholar]
  10. Hanada S., Takaichi S., Matsuura K., Nakamura K. 2002; Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 52:187–193[PubMed] [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. 1982; Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver loaded ion exchanger. J Liq Chromatogr 5:2359–2367 [View Article]
    [Google Scholar]
  14. Liu Y., Xu C.-J., Jiang J.-T., Liu Y.-H., Song X.-F., Li H., Liu Z.-P. 2010; Catellibacterium aquatile sp. nov., isolated from fresh water, and emended description of the genus Catellibacterium Tanaka et al. 2004. Int J Syst Evol Microbiol 60:2027–2031 [View Article][PubMed]
    [Google Scholar]
  15. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [View Article]
    [Google Scholar]
  16. Ohta H., Hattori T. 1983; Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 49:429–446[PubMed]
    [Google Scholar]
  17. Pfennig N., Trüper H. G. 1974; The phototrophic bacteria. In Bergey’s Manual of Determinitive Bacteriology, 8th edn. pp. 24–75 Edited by Buchanan R. E., Gibbons N. E. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  19. Sambrook J., Russell D. W. 2001 Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  21. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetic analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  23. Tanaka Y., Hanada S., Manome A., Tsuchida T., Kurane R., Nakamura K., Kamagata Y. 2004; Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth. Int J Syst Evol Microbiol 54:955–959 [View Article][PubMed]
    [Google Scholar]
  24. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  25. Wang B. Z., Guo P., Hang B. J., Li L., He J., Li S. P. 2009; Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product. Appl Environ Microbiol 75:5496–5500 [View Article][PubMed]
    [Google Scholar]
  26. Wang B.-Z., Guo P., Zheng J.-W., Hang B.-J., Li L., He J., Li S.-P. 2011; Sphingobium wenxiniae sp. nov., a synthetic pyrethroids (SP)-degrading bacterium isolated from activated sludge in a SP-manufacturing wastewater treatment facility. Int J Syst Evol Microbiol 61:1776–1780 [View Article][PubMed]
    [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  28. Weed Science Society of America 1994 Herbicide Handbook, 7th edn. pp. 10–59 Champaign, IL: Weed Science Society of America;
    [Google Scholar]
  29. Zheng J.-W., Chen Y.-G., Zhang J., Ni Y.-Y., Li W.-J., He J., Li S.-P. 2011; Description of Catellibacterium caeni sp. nov., reclassification of Rhodobacter changlensis Anil Kumar et al. 2007 as Catellibacterium changlense comb. nov. and emended description of the genus Catellibacterium . Int J Syst Evol Microbiol 61:1921–1926 [View Article]
    [Google Scholar]
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