1887

Abstract

A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12, was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90 % of added propanil (100 mg l) within 3 days of incubation. Growth occurred in the presence of 0–4.5 % (w/v) NaCl (optimum 0.5 %), at 10–40 °C (optimum 28 °C) and at pH 5.5–10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (Cω6 and/or Cω7). The genomic DNA G+C content of strain Y12 was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12 was a member of the genus , as it showed highest sequence similarities to DCA-1 (99.1 %) and <96.0 % similarities with other species of the genus . Strain Y12 showed low DNA–DNA relatedness values with DCA-1. Based on phenotypic, genotypic and phylogenetic properties, strain Y12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y12 ( = CCTCC AB 2010218  = KCTC 23298). An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029819-0
2012-03-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/3/495.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029819-0&mimeType=html&fmt=ahah

References

  1. Anil Kumar P. , Srinivas T. N. R. , Sasikala Ch. , Ramana Ch. V. . ( 2007; ). Rhodobacter changlensis sp. nov., a psychrotolerant, phototrophic alphaproteobacterium from the Himalayas of India. . Int J Syst Evol Microbiol 57:, 2568–2571. [CrossRef] [PubMed]
    [Google Scholar]
  2. Buck J. D. . ( 1982; ). Nonstaining (KOH) method for determination of gram reactions of marine bacteria. . Appl Environ Microbiol 44:, 992–993.[PubMed]
    [Google Scholar]
  3. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. , Pirouz T. , Goodfellow M. , Minnikin D. E. . ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230.[PubMed] [CrossRef]
    [Google Scholar]
  5. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  6. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  8. Gregersen T. . ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 123–127. [CrossRef]
    [Google Scholar]
  9. Guo P. , Wang B. Z. , Hang B. J. , Li L. , Ali S. W. , He J. , Li S. . ( 2009; ). Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase. . Int Biodeterior Biodegradation 63:, 1107–1112. [CrossRef]
    [Google Scholar]
  10. Hanada S. , Takaichi S. , Matsuura K. , Nakamura K. . ( 2002; ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. . Int J Syst Evol Microbiol 52:, 187–193.[PubMed] [CrossRef]
    [Google Scholar]
  11. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver loaded ion exchanger. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  14. Liu Y. , Xu C.-J. , Jiang J.-T. , Liu Y.-H. , Song X.-F. , Li H. , Liu Z.-P. . ( 2010; ). Catellibacterium aquatile sp. nov., isolated from fresh water, and emended description of the genus Catellibacterium Tanaka et al. 2004. . Int J Syst Evol Microbiol 60:, 2027–2031. [CrossRef] [PubMed]
    [Google Scholar]
  15. Mandel M. , Marmur J. . ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B:, 195–206. [CrossRef]
    [Google Scholar]
  16. Ohta H. , Hattori T. . ( 1983; ). Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. . Antonie van Leeuwenhoek 49:, 429–446.[PubMed]
    [Google Scholar]
  17. Pfennig N. , Trüper H. G. . ( 1974; ). The phototrophic bacteria. . In Bergey’s Manual of Determinitive Bacteriology, , 8th edn., pp. 24–75. Edited by Buchanan R. E. , Gibbons N. E. . . Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  18. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  19. Sambrook J. , Russell D. W. . ( 2001; ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  20. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  21. Suzuki M. , Nakagawa Y. , Harayama S. , Yamamoto S. . ( 2001; ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:, 1639–1652. [CrossRef] [PubMed]
    [Google Scholar]
  22. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetic analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tanaka Y. , Hanada S. , Manome A. , Tsuchida T. , Kurane R. , Nakamura K. , Kamagata Y. . ( 2004; ). Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth. . Int J Syst Evol Microbiol 54:, 955–959. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tindall B. J. , Rosselló-Móra R. , Busse H.-J. , Ludwig W. , Kämpfer P. . ( 2010; ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef] [PubMed]
    [Google Scholar]
  25. Wang B. Z. , Guo P. , Hang B. J. , Li L. , He J. , Li S. P. . ( 2009; ). Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product. . Appl Environ Microbiol 75:, 5496–5500. [CrossRef] [PubMed]
    [Google Scholar]
  26. Wang B.-Z. , Guo P. , Zheng J.-W. , Hang B.-J. , Li L. , He J. , Li S.-P. . ( 2011; ). Sphingobium wenxiniae sp. nov., a synthetic pyrethroids (SP)-degrading bacterium isolated from activated sludge in a SP-manufacturing wastewater treatment facility. . Int J Syst Evol Microbiol 61:, 1776–1780. [CrossRef] [PubMed]
    [Google Scholar]
  27. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  28. Weed Science Society of America ( 1994; ). Herbicide Handbook, , 7th edn., pp. 10–59. Champaign, IL:: Weed Science Society of America;.
    [Google Scholar]
  29. Zheng J.-W. , Chen Y.-G. , Zhang J. , Ni Y.-Y. , Li W.-J. , He J. , Li S.-P. . ( 2011; ). Description of Catellibacterium caeni sp. nov., reclassification of Rhodobacter changlensis Anil Kumar et al. 2007 as Catellibacterium changlense comb. nov. and emended description of the genus Catellibacterium . . Int J Syst Evol Microbiol 61:, 1921–1926. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029819-0
Loading
/content/journal/ijsem/10.1099/ijs.0.029819-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error