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Abstract

A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12, was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90 % of added propanil (100 mg l) within 3 days of incubation. Growth occurred in the presence of 0–4.5 % (w/v) NaCl (optimum 0.5 %), at 10–40 °C (optimum 28 °C) and at pH 5.5–10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (Cω6 and/or Cω7). The genomic DNA G+C content of strain Y12 was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12 was a member of the genus , as it showed highest sequence similarities to DCA-1 (99.1 %) and <96.0 % similarities with other species of the genus . Strain Y12 showed low DNA–DNA relatedness values with DCA-1. Based on phenotypic, genotypic and phylogenetic properties, strain Y12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Y12 ( = CCTCC AB 2010218  = KCTC 23298). An emended description of the genus is also presented.

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2012-03-01
2020-08-10
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