1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue 1

sp. nov., isolated from seawater, and emended description of the genus Uchino 2002 Free

Abstract

A Gram-stain-negative, aerobic, motile, ovoid bacterial strain, designated HDW-19, was isolated from seawater of the west coast of Korea and subjected to a polyphasic taxonomic study. Strain HDW-19 grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2–3 % (w/v) NaCl. Bacteriochlorophyll was not produced by strain HDW-19. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain HDW-19 clustered with IAM 12616, with which it shared 96.4 % similarity. A neighbour-joining phylogenetic tree based on gene sequences showed that strain HDW-19 also clustered with the type strain of , sharing 83.0 % similarity. Strain HDW-19 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, three unidentified aminophospholipids and two unidentified aminolipids. The DNA G+C content of strain HDW-19 was 60.9 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, showed that strain HDW-19 can be differentiated from . On this basis, strain HDW-19 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HDW-19 ( = KCTC 23043  = CCUG 58879).

  • Published Online:
Funding
This study was supported by the:
  • the Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
  • Ministry of Education, Science and Technology (MEST) of the Republic of Korea
© 2012 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029769-0
2012-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/100.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029769-0&mimeType=html&fmt=ahah

References

  1. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  2. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
     [Google Scholar]
  3. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  4. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  5. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
     [Google Scholar]
  6. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  7. Rüger H.-J., Höfle M. G. 1992; Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev.. Int J Syst Bacteriol 42:133–143 [View Article][PubMed]
     [Google Scholar]
  8. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  9. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  10. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  11. Uchino Y., Hamada T., Yokota A. 2002; Proposal of Pseudorhodobacter ferrugineus gen. nov., comb. nov., for a non-photosynthetic marine bacterium, Agrobacterium ferrugineum, related to the genus Rhodobacter . J Gen Appl Microbiol 48:309–319 [View Article][PubMed]
     [Google Scholar]
  12. Yamamoto S., Harayama S. 1995; PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61:1104–1109[PubMed]
     [Google Scholar]
  13. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
     [Google Scholar]
  14. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
     [Google Scholar]
  15. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [View Article][PubMed]
     [Google Scholar]
  16. Yoon J.-H., Lee S. Y., Kang S. J., Lee C. H., Oh T. K. 2007; Pseudoruegeria aquimaris gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:542–547 [View Article][PubMed]
     [Google Scholar]
  17. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad’on N., Gorlenko V. M., Kompantseva E. I., Drews G. 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44:427–434 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029769-0
Loading
Pseudorhodobacter aquimaris sp. nov., isolated from seawater, and emended description of the genus Pseudorhodobacter Uchino et al. 2002
Int J Syst Evol Microbiol 62, 100 (2012); https://doi.org/10.1099/ijs.0.029769-0
/content/journal/ijsem/10.1099/ijs.0.029769-0
/content/journal/ijsem/10.1099/ijs.0.029769-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error