1887

Abstract

Obligately thermophilic, aerobic, proteolytic, endospore-forming strain N-3 was isolated from a high-temperature oilfield in Lithuania. 16S rRNA gene sequence analysis placed this strain in genetic group 5 of the endospore formers. appeared to be the closest phylogenetic neighbour (99·4 % sequence similarity). The G+C content of strain N-3 was 52·5 mol% and matched the range established for the genus . Studies of DNA–DNA relatedness and morphological and physiological analyses enabled strain N-3 to be described as a member of the genus , but could not assign this strain to any other known species of this genus. Results of this polyphasic study allowed characterization of strain N-3 as a novel species in the genus sp. nov. This species can be distinguished from and on the basis of 16S rRNA gene PCR-RFLP assays with the restriction endonucleases I, III and I. The type strain of the novel species is N-3 (=DSM 15325=VKM B-2294).

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2004-11-01
2019-10-14
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vol. , part 6, pp. 1991–1995

Graphs showing the effects of temperature, pH and salinity on the growth rate of strain N-3 are available to download. [PDF](257KB)



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