1887

Abstract

Two strains of curved bacteria, 7-1 and 7-2, isolated from well water, were phylogenetically examined to determine their taxonomic position. Strain 7-1 is a Gram-negative, slightly curved rod. Analysis of the 16S rRNA gene sequence showed that strain 7-1 formed a cluster with [] and [] . It has some similar characteristics to [] and [] , but has sufficient distance to separate it from other genera. DNA–DNA hybridization analysis, as well as chemotaxonomic and morphological studies, demonstrated that strain 7-1, [] and [] belong to a new genus, gen. nov. Strain 7-1 (=IAM 15033=ATCC BAA-807) is classified as the type strain of gen. nov., sp. nov., and [] and [] are classified as comb. nov. and comb. nov., respectively. Strain 7-2 is a Gram-negative spirillum. Phylogenetic study based on the 16S rRNA gene sequences showed that it formed a cluster with the members of the genus , [] and [] . The classification is therefore proposed of strain 7-2 (=IAM 15032=ATCC BAA-806) as the type strain of sp. nov., and [] and [] are transferred to the genus as comb. nov. and comb. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02975-0
2004-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/6/ijs542223.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02975-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Aragno, M. & Schlegel, H. G. ( 1978; ). Aquaspirillum autotrophicum, a new species of hydrogen-oxidizing, facultatively autotrophic bacteria. Int J Syst Bacteriol 28, 112–116.[CrossRef]
    [Google Scholar]
  3. Baldani, J. I., Baldani, V. L. D., Seldin, L. & Döbereiner, J. ( 1986; ). Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium. Int J Syst Bacteriol 36, 86–93.[CrossRef]
    [Google Scholar]
  4. Baldani, J. I., Pot, B., Kirchhof, G. & 8 other authors ( 1996; ). Emended description of Herbaspirillum; inclusion of [Pseudomonas] rubrisubalbicans, a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3. Int J Syst Bacteriol 46, 802–810.[CrossRef]
    [Google Scholar]
  5. Ding, L. & Yokota, A. ( 2002; ). Phylogenetic analysis of the genus Aquaspirillum based on 16S rRNA gene sequences. FEMS Microbiol Lett 212, 165–169.[CrossRef]
    [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  7. Hamana, K., Sakane, T. & Yokota, A. ( 1994; ). Polyamine analysis of the genera Aquaspirillum, Magnetospirillum, Oceanospirillum and Spirillum. J Gen Appl Microbiol 40, 75–82.[CrossRef]
    [Google Scholar]
  8. Hiraishi, A. ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef]
    [Google Scholar]
  9. Hiraishi, A., Hoshino, Y. & Satoh, T. ( 1991; ). Rhodoferax fermentens gen. nov., sp. nov., a phototrophic purple nonsulfur bacterium previously referred to as the “Rhodocyclus gelatinosus-like” group. Arch Microbiol 155, 330–336.
    [Google Scholar]
  10. Hylemon, P. B., Wells, J. S., Jr, Krieg, N. R. & Jannasch, H. W. ( 1973; ). The genus Spirillum: a taxonomic study. Int J Syst Bacteriol 23, 340–380.[CrossRef]
    [Google Scholar]
  11. IAM ( 1998; ). Catalogue of Strains, pp. 277–285. Tokyo: The Microbiology Research Foundation.
  12. Irgens, R. L., Gosink, J. J. & Staley, J. T. ( 1996; ). Polaromonas vacuolata gen. nov., sp. nov., a psychrophilic, marine, gas vacuolate bacterium from Antarctica. Int J Syst Bacteriol 46, 822–826.[CrossRef]
    [Google Scholar]
  13. Kersters, K., Ludwig, W., Vancanneyt, M., De Vos, P., Gillis, M. & Schleifer, K.-H. ( 1996; ). Recent change in the classification of the pseudomonads: an overview. Syst Appl Microbiol 19, 465–477.[CrossRef]
    [Google Scholar]
  14. Kirchhof, G., Eckert, B., Stoffels, M., Baldani, J. I., Reis, V. M. & Hartmann, A. ( 2001; ). Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. Int J Syst Evol Microbiol 51, 157–168.
    [Google Scholar]
  15. Krieg, N. R. ( 1984; ). Genus Aquaspirillum Hylemon, Wells, Krieg and Jannasch 1973, 361AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 72–90. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Leifson, E. ( 1962; ). The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas. Int Bull Bacteriol Nomencl Taxon 12, 161–170.
    [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  18. Meyer, S. A. & Schleifer, K. H. ( 1978; ). Deoxyribonucleic acid reassociation in the classification of coagulase-positive staphylococci. Arch Microbiol 117, 183–188.[CrossRef]
    [Google Scholar]
  19. Pot, B., Willems, A., Gillis, M. & De Ley, J. ( 1992; ). Intra- and intergeneric relationships of the genus Aquaspirillum: Prolinoborus, a new genus for Aquaspirillum fasciculus, with the species Prolinoborus fasciculus comb. nov. Int J Syst Bacteriol 42, 44–57.[CrossRef]
    [Google Scholar]
  20. Reinhold-Hurek, B. & Hurek, T. ( 1998; ). Life in grasses: diazotrophic endophytes. Trends Microbiol 6, 139–144.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Sakane, T. & Yokota, A. ( 1994; ). Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum, Magnetospirillum and Oceanospirillum. Syst Appl Microbiol 17, 128–134.[CrossRef]
    [Google Scholar]
  23. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  24. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  25. Stoltzfus, J. R., So, R., Malarvithi, P. P., Ladha, J. K. & de Bruijin, F. J. ( 1997; ). Isolation of endophytic bacteria from rice and assessment of their potential for supplying rice with biologically fixed nitrogen. Plant Soil 194, 25–36.[CrossRef]
    [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Uchino, Y., Yokota, A. & Sugiyama, J. ( 1997; ). Phylogenetic position of the marine subdivision of Agrobacterium species based on 16S rRNA sequence analysis. J Gen Appl Microbiol 43, 243–247.[CrossRef]
    [Google Scholar]
  28. Valverde, A., Velazquez, E., Gutierrez, C., Cervantes, E., Ventosa, A. & Igual, J. M. ( 2003; ). Herbaspirillum lusitanum sp. nov., a novel nitrogen-fixing bacterium associated with root nodules of Phaseolus vulgaris. Int J Syst Evol Microbiol 53, 1979–1983.[CrossRef]
    [Google Scholar]
  29. Wen, A., Fegan, M., Hayward, C., Chakraborty, S. & Sly, L. I. ( 1999; ). Phylogenetic relationships among members of the Comamonadaceae, and description of Delftia acidovorans (den Dooren de Jong 1926 and Tamaoka et al. 1987) gen. nov., comb. nov. Int J Syst Bacteriol 49, 567–576.[CrossRef]
    [Google Scholar]
  30. Zehr, J. P. & McReynolds, L. A. ( 1989; ). Use of degenerate oligonucleotides for amplification of the nifH gene from the marine cyanobacterium Trichodesmium thiebautii. Appl Environ Microbiol 55, 2522–2526.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02975-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02975-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error