1887

Abstract

A Gram-negative, non-flagellated, non-spore-forming bacterium, designated YCS-6, that was motile by gliding, was isolated from seawater on the southern coast of Korea. Strain YCS-6 grew optimally at 30 °C and with 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YCS-6 fell within the genus and was most closely associated with B-I, with which the isolate exhibited 95.9 % 16S rRNA gene sequence similarity. Sequence similarity between strain YCS-6 and other members of the genus was 93.8–95.7 %. Strain YCS-6 contained menaquinone-6 (MK-6) as the predominant respiratory quinone and iso-C, summed feature 3 (iso-C 2-OH and/or Cω7), iso-C 3-OH and iso-C G as the major fatty acids. The DNA G+C content was 32.7 mol%. Differential phenotypic properties and phylogenetic distinctiveness distinguished strain YCS-6 from all other members of the genus . On the basis of our phenotypic, chemotaxonomic and phylogenetic data, strain YCS-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YCS-6 ( = KCTC 23423  = CCUG 60527).

Funding
This study was supported by the:
  • 21C Frontier Program of Microbial Genomics and Applications (Award 11-2008-00-002-00)
  • Program for Collection, Management and Utilization of Biological Resources (Award M10867010003)
  • Ministry of Education, Science and Technology (MEST), Republic of Korea
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2012-01-01
2024-12-07
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References

  1. Bernardet J.-F., Nakagawa Y. 2006; An introduction to the family Flavobacteriaceae . In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol. 7 pp. 455–480 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  2. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  4. Choi D. H., Kim Y.-G., Hwang C. Y., Yi H., Chun J., Cho B. C. 2006; Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56:635–640 [View Article][PubMed]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Euzéby J. P. 1997; List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [View Article][PubMed]
    [Google Scholar]
  7. Frette L., Jørgensen N. O. G., Irming H., Kroer N. 2004; Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54:519–524 [View Article][PubMed]
    [Google Scholar]
  8. Heindl H., Wiese J., Imhoff J. F. 2008; Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea. Int J Syst Evol Microbiol 58:542–547 [View Article][PubMed]
    [Google Scholar]
  9. Jung S.-Y., Oh T.-K., Yoon J.-H. 2006; Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56:1577–1581 [View Article][PubMed]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  11. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  12. Lee Y. S., Baik K. S., Park S. Y., Kim E. M., Lee D.-H., Kahng H.-Y., Jeon C. O., Jung J. S. 2009; Tenacibaculum crassostreae sp. nov., isolated from the Pacific oyster, Crassostrea gigas . Int J Syst Evol Microbiol 59:1609–1614 [View Article][PubMed]
    [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  14. Piñeiro-Vidal M., Riaza A., Santos Y. 2008; Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58:21–25 [View Article][PubMed]
    [Google Scholar]
  15. Reichenbach H. 1992; The order Cytophagales . In The Prokaryotes, 2nd edn. vol. 4 pp. 3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
    [Google Scholar]
  16. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  17. Sheu S.-Y., Lin K.-Y., Chou J.-H., Chang P.-S., Arun A. B., Young C.-C., Chen W.-M. 2007; Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57:1148–1153 [View Article][PubMed]
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  19. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  21. Wang J.-T., Chou Y.-J., Chou J.-H., Chen C. A., Chen W.-M. 2008; Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone Aiptasia pulchella . Int J Syst Evol Microbiol 58:761–766 [View Article][PubMed]
    [Google Scholar]
  22. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  23. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
    [Google Scholar]
  25. Yoon J.-H., Kang S.-J., Oh T.-K. 2005; Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:793–798 [View Article][PubMed]
    [Google Scholar]
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