1887

Abstract

Seven phylloplane yeast strains that were collected in the Arrábida Natural Park, Portugal, and identified preliminarily as , the anamorphic stage of , were investigated. In contrast to , these isolates produced a brownish pigment when grown on potato dextrose agar. The pigment caused darkening of the cultures and diffused into the culture medium. Mating studies revealed that the Arrábida isolates did not react with the different mating types of , but were sexually compatible with them and produced mycelium with clamp connections, haustoria and transversally septate basidia that ejected the basidiospores. Various taxonomic criteria that were evaluated during the present study and comparison with other sexual taxa of the Tremellales indicated that this teleomorph should be classified in a novel genus. Therefore, gen. nov., sp. nov. (type strain, PYCC 5690=CBS 9648) is proposed. In addition, during the course of this investigation, a member of a novel species, sp. nov. (type strain, PYCC 4534=CBS 2013), was found among collection isolates that were identified formerly as . In molecular phylogenetic analysis of the D1/D2 domains of the 26S rDNA and the internal transcribed spacer region, the two taxa were found to be closely related, but distinct at the species level.

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2004-05-01
2020-11-30
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References

  1. Bai F.-Y., Takashima M., Nakase T. 2001; Phylogenetic analysis of strains originally assigned to Bullera variabilis : descriptions of Bullera pseudohuiaensis sp.nov., Bullera komagatae sp. nov. and Bullera pseudoschimicola sp. nov. Int J Syst Evol Microbiol 51:2177–2187 [CrossRef]
    [Google Scholar]
  2. Boekhout T., Nakase T. 1998; Bullera Derx. In The Yeasts, a Taxonomic Study . , 4th edn. pp  731–741 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
  3. Boekhout T., Fonseca A., Batenburg-van der Vegte W. H. 1991; Bulleromyces genus novum (Tremellales), a teleomorph for Bullera alba , and the occurrence of mating in Bullera variabilis . Antonie van Leeuwenhoek 59:81–93 [CrossRef]
    [Google Scholar]
  4. Chen C.-J. 1998; Morphological and molecular studies in the genus Tremella . Bibl Mycol 174:1–225
    [Google Scholar]
  5. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [CrossRef]
    [Google Scholar]
  6. Fonseca A. 1992; Utilization of tartaric acid and related compounds by yeasts: taxonomic implications. Can J Microbiol 38:1242–1251 [CrossRef]
    [Google Scholar]
  7. Huelsenbeck J. P., Ronquist F. 2001; mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 [CrossRef]
    [Google Scholar]
  8. Inácio J., Pereira P., Carvalho M., Fonseca Á., Amaral-Collaço M. T., Spencer-Martins I. 2002; Estimation and diversity of phylloplane mycobiota on selected plants in a Mediterranean-type ecosystem in Portugal. Microbial Ecol 44:344–353 [CrossRef]
    [Google Scholar]
  9. Kurtzman C. P., Smiley M. J., Johnson C. J., Wickerham L. J., Fuson G. B. 1980; Two new and closely related heterothallic species, Pichia amylophila and Pichia mississippiensis : characterization by hybridization and deoxyribonucleic acid reassociation. Int J Syst Bacteriol 30:208–216 [CrossRef]
    [Google Scholar]
  10. Larget B., Simon D. L. 1999; Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Mol Biol Evol 16:750–759 [CrossRef]
    [Google Scholar]
  11. Nakase T. 2000; Expanding world of ballistosporous yeasts: distribution in the phyllosphere, systematics and phylogeny. J Gen Appl Microbiol 46:189–216 [CrossRef]
    [Google Scholar]
  12. Sampaio J. P. 1999; Utilization of low molecular weight aromatic compounds by heterobasidiomycetous yeasts: taxonomic implications. Can J Microbiol 45:491–512 [CrossRef]
    [Google Scholar]
  13. Sampaio J. P., Gadanho M., Santos S., Duarte F. L., Pais C., Fonseca Á., Fell J. W. 2001; Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium : Rhodosporidium kratochvilovae and related anamorphic species. Int J Syst Evol Microbiol 51:687–697
    [Google Scholar]
  14. Sampaio J. P., Weiß M., Gadanho M., Bauer R. 2002; New taxa in the Tremellales: Bulleribasidium oberjochense gen et sp. nov., Papiliotrema bandonii gen. et sp. nov. and Fibulobasidium murrhardtense sp. nov.. Mycologia 94:873–887 [CrossRef]
    [Google Scholar]
  15. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. 2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517 [CrossRef]
    [Google Scholar]
  16. Takashima M., Deak T., Nakase T. 2001; Emendation of Dioszegia with redescription of Dioszegia hungarica and two new combinations, Dioszegia aurantiaca and Dioszegia crocea . J Gen Appl Microbiol 47:75–84 [CrossRef]
    [Google Scholar]
  17. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study , 4th edn. pp  77–100 Edited by Kurtzman C. P, Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
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