1887

Abstract

A Gram-negative, facultatively anaerobic, chemo-organotrophic, non-pigmented, slow-growing bacterium was isolated from acidic peat and designated strain TPB6017. Cells of this strain were long rods that multiplied by normal cell division and were motile by means of a single flagellum. Cells grew under reduced oxygen tension and under anoxic conditions and were able to ferment sugars and several polysaccharides, including amorphous and crystalline cellulose. Strain TPB6017 was a psychrotolerant acidophile capable of growth between pH 3.0 and 7.5 (optimum 4.5–5.0) and at 4–35 °C (optimum 20–28 °C). It was extremely sensitive to salt stress; growth was inhibited at NaCl concentrations above 0.1 % (w/v). The major fatty acids were iso-C and iso-Cω9; the polar lipids were phosphatidylethanolamine and a number of phospholipids and aminophospholipids with an unknown structure. The quinone was MK-8. The DNA G+C content was 57.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that strain TPB6017 was a member of subdivision 1 of the phylum and belonged to a phylogenetic lineage defined by the acidophilic aerobic chemo-organotroph (92.3 % sequence similarity). However, cell morphology, type of flagellation, the absence of pigment, differences in fatty acid and polar lipid composition, possession of a cellulolytic capability, inability to grow under fully oxic conditions and good growth in anoxic conditions distinguished strain TPB6017 from . Therefore, it is proposed that strain TPB6017 represents a novel acidobacterium species in a new genus, gen. nov., sp. nov.; strain TPB6017 ( = DSM 23630 = VKM B-2570) is the type strain.

Funding
This study was supported by the:
  • , Program ‘Molecular and Cell Biology’ of Russian Academy of Sciences
  • , RosNauka , (Award 02.740.11.0023)
  • , Russian Fund of Basic Research , (Award 09-04-00004)
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2012-02-01
2020-09-29
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