A novel thermophilic, microaerophilic, sulfur-reducing bacterium designated strain St55BT was isolated from a sulfide chimney in the hydrothermal field of Suiyo Seamount (Izu-Bonin Arc, Western Pacific). Cells of the isolate were rod-shaped and tended to form a chain-link circular structure (a rotund body) at exponential phase under good growth conditions. The isolate was a chemoheterotroph requiring yeast extract for growth. Although strain St55BT used oxygen as an electron acceptor, it could not form colonies in an oxygen concentration of more than 5 % (v/v). The isolate also used nitrate, nitrite or elemental sulfur in the absence of oxygen. A phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolate was closely related to Oceanithermus profundus, belonging to the phylum ‘Deinococcus–Thermus’ (sequence similarity 99·5 %). However, strain St55BT differed from O. profundus in terms of usage of electron donors, cellular fatty acid profile and DNA G+C content. In addition, a DNA–DNA hybridization test indicated low relatedness between the isolate and O. profundus. For the reasons given above, the name Oceanithermus desulfurans sp. nov. is proposed for strain St55BT (=NBRC 100063T=DSM 15757T).
AlainK.,
MarteinssonV. T.,
MiroshnichenkoM. L.,
Bonch-OsmolovskayaE. A.,
PrieurD.,
BirrienJ.-L.2002b; Marinitoga piezophila sp. nov., a rod-shaped, thermo-piezophilic bacterium isolated under high hydrostatic pressure from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 52:1331–1339[CrossRef]
CorreE.,
ReysenbachA. L.,
PrieurD.2001; Epsilon-proteobacterial diversity from a deep-sea hydrothermal vent on the Mid-Atlantic Ridge. FEMS Microbiol Lett 205:329–335
Da CostaM. S.,
RaineyF. A.2001; Family I. Thermaceae fam. nov. In Bergey's Manual of Systematic Bacteriology: the Archaea and the Deeply Branching and Phototrophic Bacteria
. , 2nd edn. vol 1 pp 403–404 Edited by
BooneD. R.,
CastenholzR. W.
New York: Springer;
DSMZ1993Catalogue of Strains , 5th edn. Braunschweig: Gesellschaft fur Biotechnologische Forschung;
HeisingS.,
RichterL.,
LudwigW.,
SchinkB.1999; Chlorobium ferrooxidans sp. nov., a phototrophic green sulfur bacterium that oxidizes ferrous iron in coculture with a “Geospirillum” sp. strain. Arch Microbiol 172:116–124[CrossRef]
HuberH.,
DillerS.,
HornC.,
RachelR.2002; Thermovibrio ruber gen. nov., sp. nov., an extremely thermophilic, chemolithoautotrophic, nitrate-reducing bacterium that forms a deep branch within the phylum Aquificae
. Int J Syst Evol Microbiol 52:1859–1865[CrossRef]
JeanthonC.,
L'HaridonS.,
CueffV.,
BantaA.,
ReysenbachA.-L.,
PrieurD.2002; Thermodesulfobacterium hydrogeniphilum sp. nov., a thermophilic, chemolithoautotrophic, sulfate-reducing bacterium isolated from a deep-sea hydrothermal vent at Guaymas Basin, and emendation of the genus Thermodesulfobacterium
. Int J Syst Evol Microbiol 52:765–772[CrossRef]
MiroshnichenkoM. L.,
L'HaridonS.,
NercessianO. & 8 other authors (2003b). Vulcanithermus mediatlanticus gen. nov., sp. nov., a novel member of the family Thermaceae from a deep-sea hot vent. Int J Syst Evol Microbiol 53:1143–1148[CrossRef]
TakaiK.,
KomatsuT.,
InagakiF.,
HorikoshiK.2001; Distribution of archaea in a black smoker chimney structure. Appl Environ Microbiol 67:3618–3629[CrossRef]
TakaiK.,
InagakiF.,
NakagawaS.,
HirayamaH.,
NunouraT.,
SakoY.,
NealsonK. H.,
HorikoshiK.2003; Isolation and phylogenetic diversity of members of previously uncultivated epsilon-Proteobacteria in deep-sea hydrothermal fields. FEMS Microbiol Lett 218:167–174
ThompsonJ. D.,
HigginsD. G.,
GibsonT. J.1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.9 other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
WeryN.,
LesongeurF.,
PignetP.,
DerennesV.,
Cambon-BonavitaM.-A.,
GodfroyA.,
BarbierG.2001; Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales , isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51:495–504