1887

Abstract

Aerobic, endospore-forming bacteria that are attributed to the genus or related genera constitute a hazard to the quality of gelatin. During repetitive extragenic palindromic DNA (rep)-PCR screening of gelatin isolates, a group of five isolates (group 1) and a group of 66 isolates (group 2) that did not match any pattern in our database were found. On the basis of 16S rDNA sequence analysis, representative strains of the different rep-PCR fingerprint types of group 1 were shown to be related most closely to species, but with sequence similarity of <97 %. Likewise, representative strains of group 2 were shown to be related most closely to species, with 16S rDNA sequence similarity of <97 %. DNA–DNA reassociation values of isolates that displayed the most divergent rep-PCR profiles revealed that strains within each group belonged to a single species, according to recommendations for species delineation. A mean fatty acid profile could be calculated for each group. Isolates within a single group had similar patterns of results in API and other phenotypic tests; no correlation of patterns of results with rep-PCR groups was seen. Physiological characterization of group 1 isolates allows their distinction from other species. Despite the weak reaction of group 2 isolates in API tests, physiological characterization allows distinction between species that react weakly in API tests. Two novel species are therefore proposed, with the names sp. nov. (type strain, LMG 21881=DSM 15866) and sp. nov. (type strain, LMG 21880=DSM 15865).

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2004-05-01
2019-10-21
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References

  1. Belduz, A. O., Dulger, S. & Demirbag, Z. ( 2003; ). Anoxybacillus gonensis sp. nov., a moderately thermophilic, xylose-utilizing, endospore-forming bacterium. Int J Syst Evol Microbiol 53, 1315–1320.[CrossRef]
    [Google Scholar]
  2. De Clerck, E. & De Vos, P. ( 2002; ). Study of the bacterial load in a gelatine production process focussed on Bacillus and related endosporeforming genera. Syst Appl Microbiol 25, 611–618.[CrossRef]
    [Google Scholar]
  3. De Clerck, E., Vanhoutte, T., Hebb, T., Geerinck, J., Devos, J. & De Vos, P. ( 2004; ). Isolation, characterization, and identification of bacterial contaminants in semi-final gelatine extracts. Appl Environ Microbiol (in press).
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Gevers, D., Huys, G. & Swings, J. ( 2001; ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205, 31–36.[CrossRef]
    [Google Scholar]
  6. Heyndrickx, M., Vauterin, L., Vandamme, P., Kersters, K. & De Vos, P. ( 1996; ). Applicability of combined amplified ribosomal DNA restriction analysis (ARDRA) patterns in bacterial phylogeny and taxonomy. J Microbiol Methods 26, 247–259.[CrossRef]
    [Google Scholar]
  7. Heyrman, J. & Swings, J. ( 2001; ). 16S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (necropolis of Carmona, Seville, Spain). Syst Appl Microbiol 24, 417–422.[CrossRef]
    [Google Scholar]
  8. Logan, N. A. & Berkeley, R. C. W. ( 1984; ). Identification of Bacillus strains using the API System. J Gen Microbiol 130, 1871–1882.
    [Google Scholar]
  9. Logan, N. A., Lebbe, L., Hoste, B. & 7 other authors ( 2000; ). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov. Int J Syst Evol Microbiol 50, 1741–1753.
    [Google Scholar]
  10. Nielsen, P., Fritze, D. & Priest, F. G. ( 1995; ). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef]
    [Google Scholar]
  11. Pearson, W. & Lipman, D. ( 1988; ). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef]
    [Google Scholar]
  12. Pikuta, E., Lysenko, A., Chuvilskaya, N., Mendrock, U., Hippe, H., Suzina, N., Nikitin, D., Osipov, G. & Laurinavichius, K. ( 2000; ). Anoxybacillus pushchinensis gen. nov., sp nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50, 2109–2117.[CrossRef]
    [Google Scholar]
  13. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  14. Priest, F. G., Goodfellow, M. & Todd, C. ( 1988; ). A numerical classification of the genus Bacillus. J Gen Microbiol 134, 1847–1882.
    [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Stackebrandt, E. & Swiderski, J. ( 2002; ). From phylogeny to systematics: the dissection of the genus Bacillus. In Applications and Systematics of Bacillus and Relatives, pp. 8–22. Edited by R. Berkeley, M. Heyndrickx, N. A. Logan and P. De Vos. Oxford: Blackwell Publishing.
  18. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  20. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  21. Versalovic, J., Schneider, M., De Bruijn, F. J. & Lupski, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  23. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
  24. Yang, P., Vauterin, L., Vancanneyt, M., Swings, J. & Kersters, K. ( 1993; ). Application of fatty acid methyl esters for the taxonomic analysis of the genus Xanthomonas. Syst Appl Microbiol 16, 47–71.
    [Google Scholar]
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vol. , part 3, pp. 941 – 946

Phylogenetic position of among other species and the type species of closely related genera.

Phylogenetic position of among a selection of other bacilli including the type species, , and the type species of closely related genera.

Fatty acid composition of strains.

Fatty acid composition of representative strains.

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