1887

Abstract

Previously, analysis of 16S rDNA sequences placed a newly discovered lineage of bacterial symbionts of arthropods in the ‘’. This symbiont lineage is associated with a number of diverse host reproductive manipulations, including induction of parthenogenesis in several parasitoid wasps (Hymenoptera: Aphelinidae). In this study, electron microscopy and phylogenetic analysis of the 16S rRNA and genes of symbionts from and are used to describe and further characterize these bacteria. Phylogenetic analyses based on these two genes showed that the symbionts are allied with the lineage within the ‘’, with their closest described relative being the acanthamoeba symbiont ‘ Amoebophilus asiaticus’. The symbionts share 97 % 16S rDNA sequence similarity with mite and tick symbionts and 88 % sequence similarity with ‘ A. asiaticus’. Electron microscopy revealed that many of the bacteria found in the ovaries of the two species contained a regular, brush-like array of microfilament-like structures that appear to be characteristic of the symbiont. Finally, the role of this bacterium in parthenogenesis induction in was confirmed. Based on phylogenetic analyses and electron microscopy, classification of the symbionts from as ‘ Cardinium hertigii’ is proposed.

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2004-05-01
2019-08-18
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