1887

Abstract

Two actinomycete strains, DSA1 and HD9, were isolated from a potato tuber and soil from a potato-cultivating field in Jeju, Korea, respectively. A comprehensive 16S rDNA analysis revealed that the isolates belong to the genus and share 97·7–98·6 % sequence similarity to species. The strains also contained typical chemotaxonomic markers of the genus : -diaminopimelic acid, alanine, glycine and glutamic acid in the cell wall peptidoglycan; mannose, glucose, galactose and ribose as whole cell sugars; diphosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as characteristic phospholipids; and the major menaquinone MK-9 (H). DNA–DNA hybridization experiments showed that the isolates represented two distinct genomic species. A number of phenotypic properties can be used to differentiate the two isolates from species. On the basis of polyphasic evidence, two novel species are proposed: sp. nov. for strain DSA1 (=KACC 20196=JCM 12205) and sp. nov. for strain HD9 (=KACC 20266=JCM 12204).

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2004-07-01
2019-10-19
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vol. , part 4, pp. 1345 - 1348

Phylogenetic tree showing the relationship of isolates DSA1 and HD9 and related actinomycete taxa. Numbers at branching points indicate percentages of occurrence based on neighbour-joining analyses of 1000 resampled dataset bootstrapped trees. Bar, 1 nucleotide substitution per 100 nucleotides.

Maximum-parsimony tree showing the relationship of isolates DSA1 and HD9 and related actinomycete taxa. Numbers at branching points indicate percentages of occurrence based on maximum-parsimony analyses of 1000 resampled dataset bootstrapped trees.

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