1887

Abstract

It was shown recently that the genus is rich in N-fixing bacteria that are associated with plants. A group of these diazotrophic isolates with identical or very similar 16S rDNA restriction patterns [designated amplified rDNA restriction analysis (ARDRA) genotypes 13, 14 and 15] was selected and a polyphasic taxonomic study was performed, which included new isolates that were recovered from rhizospheres, rhizoplanes or internal tissues of maize, sugarcane and coffee plants. Morphological, physiological and biochemical features, as well as multi-locus enzyme electrophoresis profiles and whole-cell protein patterns, of 20 strains were analysed. In addition, analysis of cellular fatty acid profiles, 16S rDNA sequence analysis and DNA–DNA reassociation experiments were performed with representative strains. The taxonomic data indicated that the strains analysed belong to a novel diazotrophic species, for which the name sp. nov. is proposed. Strain MTl-641 (=ATCC BAA-744=CIP 107921), isolated from the rhizosphere of maize, was designated as the type strain. was found as an endophyte of plants grown in regions with climates ranging from semi-hot subhumid to hot humid, but not from plants grown in regions with semi-hot or hot dry climates. Moreover, was isolated from rhizospheres and plants growing in soils with pH values in the range 4·5–7·1, but not from soils with pH values higher than 7·5.

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2004-07-01
2019-10-16
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Supplements

vol. , part 4, pp. 1165 – 1172

16S rDNA genotypes and restriction patterns of and other related diazotrophic and non-N -fixing species.

Phylogenetic tree showing the relationships of with all known species.

Relationships among isolates as determined by multi-locus enzyme electrophoresis.

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