1887

Abstract

A Gram-stain-negative, motile, non-spore-forming and short rod- or rod-shaped bacterial strain, T-w6, was isolated from seawater of an oyster farm in the South Sea, Korea. Strain T-w6 grew optimally at 25 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain T-w6 joined the cluster comprising species with a bootstrap resampling value of 90.8 %, and this cluster joined the clade comprising members of the genera and with a bootstrap resampling value of 100 %. Strain T-w6 exhibited 16S rRNA gene sequence similarity of 95.9 and 96.6 % to the type strains of and , respectively. Strain T-w6 and the type strains of and had Q-8 as the predominant ubiquinone and iso-C 2-OH and/or Cω7, Cω7 and C as the major fatty acids. The major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain T-w6 was 56.6 mol%. Mean DNA–DNA relatedness of strain T-w6 with DSM 15406 and KCTC 12522 was 13 and 10 %, respectively. Phenotypic properties of strain T-w6 demonstrated that this strain could be distinguished from the other species. On the basis of the data presented, strain T-w6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is T-w6 ( = KCTC 23422  = CCUG 60525). An emended description of the genus is also presented.

Funding
This study was supported by the:
  • 21C Frontier Program of Microbial Genomics and Applications
  • Ministry of Education, Science and Technology (MEST) of the Republic of Korea (Award 11-2008-00-002-00)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029496-0
2011-12-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/12/2880.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029496-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp. 1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  2. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  3. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 49:25–68 [View Article]
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  7. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  8. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  9. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  10. Murray R. G. E., Doetsch R. N., Robinow F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Park S.-J., Kang C.-H., Nam Y.-D., Bae J.-W., Park Y.-H., Quan Z.-X., Moon D.-S., Kim H.-J., Roh D.-H., Rhee S.-K. 2006; Oceanisphaera donghaensis sp. nov., a halophilic bacterium from the East Sea, Korea. Int J Syst Evol Microbiol 56:895–898 [View Article][PubMed]
    [Google Scholar]
  12. Romanenko L. A., Schumann P., Zhukova N. V., Rohde M., Mikhailov V. V., Stackebrandt E. 2003; Oceanisphaera litoralis gen. nov., sp. nov., a novel halophilic bacterium from marine bottom sediments. Int J Syst Evol Microbiol 53:1885–1888 [View Article][PubMed]
    [Google Scholar]
  13. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101.. Newark, DE: MIDI Inc.;
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  16. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
    [Google Scholar]
  17. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  18. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  19. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  20. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029496-0
Loading
/content/journal/ijsem/10.1099/ijs.0.029496-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error