1887

Abstract

During a study of endophytic bacteria from coastal dune plants, a bacterial strain, designated YC6881, was isolated from the root of collected from the coastal dune areas of Namhae Island, Korea. The bacterium was found to be Gram-staining-negative, motile, halophilic and heterotrophic with a single polar flagellum. Strain YC6881 grew at temperatures of 4–37 °C (optimum, 28–32 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0), and at NaCl concentrations in the range of 0–7.5 % (w/v) (optimum, 4–5 % NaCl). Strain YC6881 was catalase- and oxidase-positive and negative for nitrate reduction. According to phylogenetic analysis using 16S rRNA gene sequences, strain YC6881 belonged to the genus and showed the highest 16S rRNA gene sequence similarity of 96.9 % to , followed by (96.3 %), (96.1 %), (95.9 %), (95.6 %) and (95.4 %). Phylogenetic analysis of strain YC6881 by , , and 16S–23S intergenic spacer (IGS) sequences all confirmed the phylogenetic arrangements obtained by using 16S rRNA gene sequences. Cross-nodulation tests showed that strain YC6881 was a symbiotic bacterium that nodulated and . The major components of the cellular fatty acids were Cω7 (53.7 %), C cyclo ω8 (12.6 %) and C (8.1 %). The DNA G+C content was 52.8 mol%. Phenotypic and physiological tests with respect to carbon source utilization, antibiotic resistance, growth conditions, phylogenetic analyses of housekeeping genes , and , and fatty acid composition could be used to discriminate strain YC6881 from other species of the genus in the same sublineage. Based on the results obtained in this study, strain YC6881 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YC6881 ( = KACC 13775 = DSM 21600).

Funding
This study was supported by the:
  • , Brain Korea (BK) 21 project
  • , Ministry of Education, Science and Technology, Korea
  • , the Ministry of Knowledge Economy, Korea
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2012-08-01
2020-10-22
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