sp. nov., an endophytic actinobacterium isolated from a surface-sterilized stem of L. Free

Abstract

A novel actinobacterium, designated YIM 65623, was isolated from a surface-sterilized stem of L. Strain YIM 65623 had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus . Growth occurred with 0–7 % (w/v) NaCl (optimum 0–3 %), at pH 5.0–9.0 (optimum pH 6.0) and at 10–37 °C (optimum 20–28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623 constituted a distinct sublineage within the genus and displayed 94.1–98.2 % sequence similarity to members of established species in the genus . However, DNA–DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623 could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623 be classified as a representative of a novel species, sp. nov. The type strain is YIM 65623 ( = DSM 45379  = CCTCC AA 209038).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award U0932601)
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2011-12-01
2024-03-29
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