1887

Abstract

A novel actinobacterium, designated YIM 65623, was isolated from a surface-sterilized stem of L. Strain YIM 65623 had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus . Growth occurred with 0–7 % (w/v) NaCl (optimum 0–3 %), at pH 5.0–9.0 (optimum pH 6.0) and at 10–37 °C (optimum 20–28 °C). Comparative 16S rRNA gene sequence analysis showed that strain YIM 65623 constituted a distinct sublineage within the genus and displayed 94.1–98.2 % sequence similarity to members of established species in the genus . However, DNA–DNA relatedness and physiological and biochemical characteristics showed that strain YIM 65623 could be differentiated from its closest phylogenetic relatives. The G+C content of the genomic DNA was 69.6 mol%. It is proposed that strain YIM 65623 be classified as a representative of a novel species, sp. nov. The type strain is YIM 65623 ( = DSM 45379  = CCTCC AA 209038).

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2011-12-01
2019-10-19
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Supplements

vol. , part 12, pp. 2933 - 2937

Cultural characteristics of sp. nov. YIM 65623 .

Fatty acid profiles of sp. nov. YIM 65623 and its closest phylogenetic neighbour.

Scanning electron micrograph of cells of sp. nov. YIM 65623 grown on ISP 2 medium for 2 weeks at 28 °C.

Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, showing the position of sp. nov. YIM 65623 among its phylogenetic neighbours within the genus .

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