RT Journal Article SR Electronic(1) A1 Rojas, Ana María A1 Rios, Jose Esteban García de los A1 Saux, Marion Fischer-Le A1 Jimenez, Pedro A1 Reche, Paloma A1 Bonneau, Sophie A1 Sutra, Laurent A1 Mathieu-Daudé, Françoise A1 McClelland, MichaelYR 2004 T1 Erwinia toletana sp. nov., associated with Pseudomonas savastanoi-induced tree knots JF International Journal of Systematic and Evolutionary Microbiology, VO 54 IS 6 SP 2217 OP 2222 DO https://doi.org/10.1099/ijs.0.02924-0 PB Microbiology Society, SN 1466-5034, AB Gram-negative bacteria were isolated from knots induced by Pseudomonas savastanoi in olive trees (Olea europaea L.). A total of nine endophytic bacterial strains were isolated, each from inside a different tree knot. Biochemical characterization indicated that all the strains belong to the family Enterobacteriaceae. Phylogenetic analyses of the 16S rRNA genes of these novel isolates revealed that they formed a homogeneous cluster within Erwinia species. DNA signatures of these isolates were identical to those described for the genus Erwinia. The strains formed a homogeneous group as shown by DNA–DNA hybridization analysis and numerical analysis of phenotypic data, clearly differentiated from all species of Erwinia with validly published names. The data provide strong evidence of the differentiation of these strains from the most closely related species. Therefore, these isolates represent a novel species, for which the name Erwinia toletana sp. nov. is proposed. The isolates are available at CFBP, CECT and ATCC. The G+C content is 52±0·5 mol%. The type strain is CFBP 6631T (=A37T=ATCC 700880T=CECT 5263T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02924-0