1887

Abstract

Three bacterial isolates from soil, capable of degradation or transformation of nitroaromatic compounds and displaying a rod–coccus growth cycle, were studied by a polyphasic approach. On the basis of 16S rRNA sequence analysis and of chemotaxonomic characteristics, such as type A3 peptidoglycan with an interpeptide bridge Ala–Thr–Ala, the major menaquinone MK-9(H) and fatty acid composition, the isolates were assigned to the genus . DNA–DNA hybridization, riboprinting and phenotypic studies revealed that the three strains constitute a single species, distinct from phylogenetically neighbouring and . A novel species, sp. nov., with the type strain G2-1 (=CCM 4924=DSM 15232) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02923-0
2004-05-01
2021-04-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540773.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02923-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer M. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  2. Cashion P., Hodder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  4. Conn H. J., Dimmick I. 1947; Soil bacteria similar in morphology to Mycobacterium and Corynebacterium . J Bacteriol 54:291–303
    [Google Scholar]
  5. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626 [CrossRef]
    [Google Scholar]
  6. DSMZ 2001 Catalogue of strains , 7th edn. Braunschweig: DSMZ;
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  8. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  9. Jahnke K. D. 1992; Basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73 [CrossRef]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Koch C., Schumann P., Stackebrandt E. 1995; Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of the genus Arthrobacter . Int J Syst Bacteriol 45:837–839 [CrossRef]
    [Google Scholar]
  12. Kotoučková L., Vavřík J., Němec M., Plocek J., Zdráhal Z. 1997; Use of immobilized cells of the strain Corynebacterium sp. for 4-nitrophenol degradation. Folia Microbiol 42:509–512 [CrossRef]
    [Google Scholar]
  13. Maidak B. L., Cole J. R., Parker C. T. Jr 11 other authors 1999; A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  15. M. I. D. I., Inc. 1999 Sherlock, Microbial Identification System, operating manual, version 3.0 Newark, DE: MIDI, Inc;
    [Google Scholar]
  16. Pospíšil S., Benada O., Kofroňová O., Petříček M., Janda L., Havlíček V. 1998; Kytococcus sedentarius (formerly Micrococcus sedentarius ) and Dermacoccus nishinomiyaensis (formerly Micrococcus nishinomiyaensis ) produce monensins, typical Streptomyces cinnamonensis metabolites. Can J Microbiol 44:1007–1011 [CrossRef]
    [Google Scholar]
  17. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  18. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  19. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  20. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Stackebrandt E., Schumann P. 2000; Introduction to the taxonomy of actinobacteria. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , 3rd edn. release 3.3. August 9 Springer; New York: http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  23. Westerberg K., Elväng A. M., Stackebrandt E., Jansson J. K. 2000; Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol. Int J Syst Evol Microbiol 50:2083–2092 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02923-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02923-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error