1887

Abstract

Three bacterial isolates from soil, capable of degradation or transformation of nitroaromatic compounds and displaying a rod–coccus growth cycle, were studied by a polyphasic approach. On the basis of 16S rRNA sequence analysis and of chemotaxonomic characteristics, such as type A3 peptidoglycan with an interpeptide bridge Ala–Thr–Ala, the major menaquinone MK-9(H) and fatty acid composition, the isolates were assigned to the genus . DNA–DNA hybridization, riboprinting and phenotypic studies revealed that the three strains constitute a single species, distinct from phylogenetically neighbouring and . A novel species, sp. nov., with the type strain G2-1 (=CCM 4924=DSM 15232) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02923-0
2004-05-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540773.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02923-0&mimeType=html&fmt=ahah

References

  1. Brosius, J., Palmer, M. L., Kennedy, J. P. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  2. Cashion, P., Hodder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. Conn, H. J. & Dimmick, I. ( 1947; ). Soil bacteria similar in morphology to Mycobacterium and Corynebacterium. J Bacteriol 54, 291–303.
    [Google Scholar]
  5. De Soete, G. ( 1983; ). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  6. DSMZ ( 2001; ). Catalogue of strains, 7th edn. Braunschweig: DSMZ.
  7. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  8. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  9. Jahnke, K. D. ( 1992; ). Basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  10. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  11. Koch, C., Schumann, P. & Stackebrandt, E. ( 1995; ). Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of the genus Arthrobacter. Int J Syst Bacteriol 45, 837–839.[CrossRef]
    [Google Scholar]
  12. Kotoučková, L., Vavřík, J., Němec, M., Plocek, J. & Zdráhal, Z. ( 1997; ). Use of immobilized cells of the strain Corynebacterium sp. for 4-nitrophenol degradation. Folia Microbiol 42, 509–512.[CrossRef]
    [Google Scholar]
  13. Maidak, B. L., Cole, J. R., Parker, C. T., Jr & 11 other authors ( 1999; ). A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27, 171–173.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. M. I. D. I., Inc. ( 1999; ). Sherlock, Microbial Identification System, operating manual, version 3.0. Newark, DE: MIDI, Inc.
  16. Pospíšil, S., Benada, O., Kofroňová, O., Petříček, M., Janda, L. & Havlíček, V. ( 1998; ). Kytococcus sedentarius (formerly Micrococcus sedentarius) and Dermacoccus nishinomiyaensis (formerly Micrococcus nishinomiyaensis) produce monensins, typical Streptomyces cinnamonensis metabolites. Can J Microbiol 44, 1007–1011.[CrossRef]
    [Google Scholar]
  17. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  18. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  19. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  21. Stackebrandt, E. & Schumann, P. ( 2000; ). Introduction to the taxonomy of actinobacteria. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community, 3rd edn, release 3.3, August 9. Springer: New York. (http://link.springer-ny.com/link/service/books/10125/).
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  23. Westerberg, K., Elväng, A. M., Stackebrandt, E. & Jansson, J. K. ( 2000; ). Arthrobacter chlorophenolicus sp. nov., a new species capable of degrading high concentrations of 4-chlorophenol. Int J Syst Evol Microbiol 50, 2083–2092.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02923-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02923-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 773 - 777

Differentiating physiological reactions of strains G2-1 , P1P and 8/3; cellular fatty acid composition of G2- 1 , P1P and 8/3, and type strains of and . [PDF](148 kB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error