1887

Abstract

A taxonomic study was performed on three strains isolated from microbial mats in Lake Fryxell, McMurdo Dry Valleys, Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the family , in which they form a distinct lineage. The isolates are Gram-negative, chemoheterotrophic, aerobic, rod-shaped cells. They are psychrophilic and yellow-pigmented, with DNA G+C contents in the range 37·8–38·9 mol%. Whole-cell fatty acid profiles revealed mainly branched fatty acids and 17 : 0 2-OH. On the basis of genotypic, phenotypic, chemotaxonomic and phylogenetic results, it is proposed that the isolates represent a novel species in a new genus, gen. nov., sp. nov. The type strain is LMG 21470 (=DSM 15749).

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2004-03-01
2019-12-14
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References

  1. Barbeyron, T., L'Haridon, S., Corre, E., Kloareg, B. & Potin, P. ( 2001; ). Zobellia galactonovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 51, 985–997.[CrossRef]
    [Google Scholar]
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  3. Bowman, J. P. & Nichols, D. S. ( 2002; ). Aequorivita gen. nov., a member of the family Flavobacteriaceae isolated from terrestrial and marine Antarctic habitats. Int J Syst Evol Microbiol 52, 1533–1541.[CrossRef]
    [Google Scholar]
  4. Bowman, J. P., McCammon, S. A., Brown, J. L., Nichols, P. D. & McMeekin, T. A. ( 1997a; ). Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47, 670–677.[CrossRef]
    [Google Scholar]
  5. Bowman, J. P., McCammon, S. A., Brown, J. L., Nichols, P. D. & McMeekin, T. A. ( 1997b; ). Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63, 3068–3078.
    [Google Scholar]
  6. Bowman, J. P., McCammon, S. A., Lewis, T., Skerratt, J. H., Brown, J. L., Nichols, D. S. & McMeekin, T. A. ( 1998; ). Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense ( Dobson et al. 1993 ) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144, 1601–1609.[CrossRef]
    [Google Scholar]
  7. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  8. Dobson, S. J., Colwell, R. R., McMeekin, T. A. & Franzmann, P. D. ( 1993; ). Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43, 77–83.[CrossRef]
    [Google Scholar]
  9. Glöckner, F. O., Fuchs, B. M. & Amann, R. ( 1999; ). Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65, 3721–3726.
    [Google Scholar]
  10. Gosink, J. J., Woese, C. R. & Staley, J. T. ( 1998; ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the CytophagaFlavobacteriumBacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef]
    [Google Scholar]
  11. Hall, T. A. ( 1999; ). bioedit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  12. Ivanova, E. P., Nedashkovskaya, O. I., Chun, J. & 7 other authors ( 2001; ). Arenibacter gen. nov., a new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 51, 1987–1995.[CrossRef]
    [Google Scholar]
  13. Johansen, J. E., Nielsen, P. & Sjøholm, C. ( 1999; ). Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49, 1231–1240.[CrossRef]
    [Google Scholar]
  14. Kirchman, D. L. ( 2002; ). The ecology of CytophagaFlavobacteria in aquatic environments. FEMS Microbiol Ecol 39, 91–100.
    [Google Scholar]
  15. McCammon, S. A. & Bowman, J. P. ( 2000; ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Saligentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef]
    [Google Scholar]
  16. Nedashkovskaya, O. I., Suzuki, M., Vysotskii, M. V. & Mikhailov, V. V. ( 2003a; ). Vitellibacter vladivostokensis gen. nov., sp. nov., a new member of the phylum CytophagaFlavobacteriumBacteroides. Int J Syst Evol Microbiol 53, 1281–1286.[CrossRef]
    [Google Scholar]
  17. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 7 other authors ( 2003b; ). Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 53, 1967–1971.[CrossRef]
    [Google Scholar]
  18. Nedashkovskaya, O. I., Kim, S. B., Han, S. K., Rhee, M. S., Lysenko, A. M., Falsen, E., Frolova, G. M., Mikhailov, V. V. & Bae, K. S. ( 2004; ). Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 54, 119–123.[CrossRef]
    [Google Scholar]
  19. Pinhassi, J., Zweifel, U. L., Hagström, A. ( 1997; ). Dominant marine bacterioplankton species found among colony-forming bacteria. Appl Environ Microbiol 63, 3359–3366.
    [Google Scholar]
  20. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  21. Raes, J. & Van de Peer, Y. ( 1999; ). ForCon: a software tool for the conversion of sequence alignments. http://www.ebi.ac.uk/embnet.news/vol6_1/ForCon/body_forcon.html
  22. Reichenbach, H. ( 1989; ). Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S. ( 2001; ). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef]
    [Google Scholar]
  25. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  26. Van Trappen, S., Mergaert, J., Van Eygen, S., Dawyndt, P., Cnockaert, M. C. & Swings, J. ( 2002; ). Diversity of 746 heterotrophic bacteria isolated from microbial mats from ten Antarctic lakes. Syst Appl Microbiol 25, 603–610.[CrossRef]
    [Google Scholar]
  27. Van Trappen, S., Mergaert, J. & Swings, J. ( 2003; ). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef]
    [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  29. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M. ( 2001; ). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322.
    [Google Scholar]
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