1887

Abstract

A novel bacterial strain, designated MY15, was isolated from a saliva sample taken from a student during a teaching experiment in China. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was most closely related to JS-08, LMG 4029 and D25 with 96.5 %, 96.3 % and 96.1 % gene sequence similarities, respectively, demonstrating that the novel strain belonged to the genus . Strain MY15 formed pale yellow colonies that turned to brown on Luria–Bertani (LB) agar and that gave off a characteristic fruity odour. Cells were Gram-staining-negative, rod-shaped and non-motile. The new isolate contained menaquinone 6 (MK-6) as the major respiratory quinone and C iso (51.2 %), C iso 3-OH (12.9 %) and C iso (10.5 %) as the dominant fatty acids. The GC content of the DNA was 34.3 mol%. On the basis of this study, based on a polyphasic taxonomic approach, strain MY15 ( = DSM 23313 = LMG 25566) represents a novel species of the genus , for which the name sp. nov. is proposed. Emended descriptions of the genus and of the species and are also given.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.029215-0
2012-04-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/4/770.html?itemId=/content/journal/ijsem/10.1099/ijs.0.029215-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B.. ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Beveridge T. J., Lawrence J. R., Murray R. G. E.. ( 2007;). Sampling and staining for light microscopy. . In Methods for General and Molecular Microbiology, pp. 19–33. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  3. Breznak J. A., Costilow R. N.. ( 1994;). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Cho S.-H., Chae S.-H., Im W.-T., Kim S. B.. ( 2011;). Myroides marinus sp. nov., a member of the family Flavobacteriaceae, isolated from seawater. . Int J Syst Evol Microbiol 61:, 938–941. [CrossRef][PubMed]
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  6. Davis J. M., Peel M. M., Gillians J. A.. ( 1979;). Colonization of an amputation site by Flavobacterium odoratum after gentamicin therapy. . Med J Aust 2:, 703–704.[PubMed]
    [Google Scholar]
  7. Ferrer C., Jakob E., Pastorino G., Juncos L. I.. ( 1995;). Right-sided bacterial endocarditis due to Flavobacterium odoratum in a patient on chronic hemodialysis. . Am J Nephrol 15:, 82–84. [CrossRef][PubMed]
    [Google Scholar]
  8. Green B. T., Green K., Nolan P. E.. ( 2001;). Myroides odoratus cellulitis and bacteremia: case report and review. . Scand J Infect Dis 33:, 932–934. [CrossRef][PubMed]
    [Google Scholar]
  9. Holmes B., Snell J. J. S., Lapage S. P.. ( 1977;). Revised description, from clinical isolates, of Flavobacterium odoratum Stutzer and Kwaschnina 1929, and designation of the neotype strain. . Int J Syst Bacteriol 27:, 330–336. [CrossRef]
    [Google Scholar]
  10. Hsueh P. R., Wu J. J., Hsiue T. R., Hsieh W. C.. ( 1995;). Bacteremic necrotizing fasciitis due to Flavobacterium odoratum. . Clin Infect Dis 21:, 1337–1338. [CrossRef][PubMed]
    [Google Scholar]
  11. Hu Z.-Y., Li Y.. ( 2007;). Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China. . Int J Syst Evol Microbiol 57:, 2572–2577. [CrossRef][PubMed]
    [Google Scholar]
  12. Hugo C. J., Bruun B., Jooste P. J.. ( 2006;). The genera Empedobacter and Myroides. . In The Prokaryotes, , 3rd edn., vol. 7, pp. 630–637. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  13. Jin G., Wang S., Yu M., Yan S., Zhang X.-H.. ( 2010;). Identification of a marine antagonistic strain JG1 and establishment of a polymerase chain reaction. . Aquacult Res 41:, 1867–1874. [CrossRef]
    [Google Scholar]
  14. Macfarlane D. E., Baum-Thureen P., Crandon I.. ( 1985;). Flavobacterium odoratum ventriculitis treated with intraventricular cefotaxime. . J Infect 11:, 233–238. [CrossRef][PubMed]
    [Google Scholar]
  15. Mesbah M., Whitman W. B.. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479:, 297–306. [CrossRef][PubMed]
    [Google Scholar]
  16. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, Technical Note 101. . Newark, DE:: MIDI Inc;.
  17. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  18. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  19. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Vancanneyt M., Segers P., Torck U., Hoste B., Bernardet J.-F., Vandamme P., Kersters K.. ( 1996;). Reclassification of Flavobacterium odoratum (Stutzer 1929) strains to a new genus, Myroides, as Myroides odoratus comb. nov. and Myroides odoratimimus sp. nov.. Int J Syst Bacteriol 46:, 926–932. [CrossRef]
    [Google Scholar]
  21. Yağcı A., Cerikçioğlu N., Kaufmann M. E., Malnick H., Söyletir G., Babacan F., Pitt T. L.. ( 2000;). Molecular typing of Myroides odoratimimus (Flavobacterium odoratum) urinary tract infections in a Turkish hospital. . Eur J Clin Microbiol Infect Dis 19:, 731–732. [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon J., Maneerat S., Kawai F., Yokota A.. ( 2006;). Myroides pelagicus sp. nov., isolated from seawater in Thailand. . Int J Syst Evol Microbiol 56:, 1917–1920. [CrossRef][PubMed]
    [Google Scholar]
  23. Zhang X.-Y., Zhang Y.-J., Chen X.-L., Qin Q.-L., Zhao D.-L., Li T.-G., Dang H.-Y., Zhang Y.-Z.. ( 2008;). Myroides profundi sp. nov., isolated from deep-sea sediment of the southern Okinawa Trough. . FEMS Microbiol Lett 287:, 108–112. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.029215-0
Loading
/content/journal/ijsem/10.1099/ijs.0.029215-0
Loading

Data & Media loading...

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error