1887

Abstract

An unknown Gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium originating from sediment was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on -diaminopimelic acid (type -Dpm-glycine), a complex mixture of saturated, monounsaturated and iso- and anteiso-methyl-branched, non-hydroxylated, long-chain cellular fatty acids and tetrahydrogenated menaquinones with eight isoprene units [MK-8(H)] as the major respiratory lipoquinone. This combination of characteristics somewhat resembled members of the suborder , but did not correspond to any currently described species. Comparative 16S rRNA gene sequencing confirmed that the unidentified coccus-shaped organism is a member of the and represents a hitherto-unknown subline related to, albeit different from, a number of taxa including , , , and . Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium originating from lake sediment be classified as a new genus and species, gen. nov., sp. nov. (type strain CCUG 47306=DSM 15745).

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2004-03-01
2020-01-27
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