1887

Abstract

An unknown Gram-positive, catalase-positive, facultatively anaerobic, non-spore-forming, coccus-shaped bacterium originating from sediment was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a cell-wall murein based on -diaminopimelic acid (type -Dpm-glycine), a complex mixture of saturated, monounsaturated and iso- and anteiso-methyl-branched, non-hydroxylated, long-chain cellular fatty acids and tetrahydrogenated menaquinones with eight isoprene units [MK-8(H)] as the major respiratory lipoquinone. This combination of characteristics somewhat resembled members of the suborder , but did not correspond to any currently described species. Comparative 16S rRNA gene sequencing confirmed that the unidentified coccus-shaped organism is a member of the and represents a hitherto-unknown subline related to, albeit different from, a number of taxa including , , , and . Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium originating from lake sediment be classified as a new genus and species, gen. nov., sp. nov. (type strain CCUG 47306=DSM 15745).

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2004-03-01
2021-03-07
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References

  1. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Keddie R. M., Kroppenstedt R. M. 1983; Lipid composition of Arthrobacter simplex , Arthrobacter tumescens and possibly related taxa. Syst Appl Microbiol 4:18–26 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Faulkner M., Keddie R. M. 1984; Menaquinone composition of some spore forming actinomycetes. Syst Appl Microbiol 5:20–29 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2. Cladistics 5:164–166
    [Google Scholar]
  5. Grip E. 1973 Skelleftefältets sulfidmalmer. In Malm i Sverige 2 , Norra Sverige Edited by Grip E., Frietsch R. Stockholm: Almqvist & Wiksell (in Swedish;
    [Google Scholar]
  6. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. 1997; Demetria terragena gen. nov., sp. nov. a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133 [CrossRef]
    [Google Scholar]
  7. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
    [Google Scholar]
  8. Jacks G., Bhattacharya P., Routh J., Martin M. T. 2003; Arsenic cycling in a covered mine tailings deposit, northern Sweden. In Geochemical Processes in Soil and Groundwater: GeoProc 2002 pp  303–309 Edited by Schulz H. D., Hadeler A. Chichester: Wiley;
    [Google Scholar]
  9. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  10. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  11. Martin K., Schumann P., Rainey F. A., Schuetze B., Groth I. 1997; Janibacter limosus gen. nov., sp. nov. a new actinomycete with meso -diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47:529–534 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Prauser H., Schumann P., Rainey F. A., Kroppenstedt R. M., Stackebrandt E. 1997; Terracoccus luteus gen. nov., sp. nov. an ll-diaminopimelic acid-containing coccoid actinomycete from soil. Int J Syst Bacteriol 47:1218–1224 [CrossRef]
    [Google Scholar]
  14. Rasmussen S. W. 1995 DNATools, a software package for DNA sequence analysis Carlsberg Laboratory; Copenhagen:
    [Google Scholar]
  15. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  16. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov. an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [CrossRef]
    [Google Scholar]
  17. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Lee K.-C., Kang S.-S., Kho Y. H., Kang K. H., Park Y.-H. 2000; Janibacter terrae sp. nov., a bacterium isolated from soil around a wastewater treatment plant. Int J Syst Evol Microbiol 50:1821–1827
    [Google Scholar]
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