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Abstract

A Gram-staining-negative, strictly aerobic bacterium, designated strain G3, was isolated from a tidal flat of the Taean coast in South Korea. Cells were moderately halotolerant and non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain G3 was observed between 15 and 40 °C (optimum 30 °C) and between pH 5.5 and 9.0 (optimum pH 6.5–7.5). Strain G3 contained Q-8 as the predominant lipoquinone and iso-C, iso-Cω9, iso-C and iso-C as the major fatty acids. The G+C content of the genomic DNA was 69.6 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain G3 formed a tight phylogenetic lineage with B1953/27.1 within the genus and was most closely related to B1953/27.1 with 98.0 % 16S rRNA gene sequence similarity. The DNA–DNA relatedness between strain G3 and B1953/27.1 was 35.2±3.3 %. On the basis of chemotaxonomic data and molecular properties, strain G3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is G3 ( = KACC 14929 = JCM 16916).

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2011-11-01
2019-10-22
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References

  1. Baik K. S. , Park S. C. , Kim M. S. , Kim E. M. , Park C. , Chun J. , Seong C. N. . ( 2008; ). Luteimonas marina sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 58:, 2904–2908. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chou J.-H. , Cho N.-T. , Arun A. B. , Young C.-C. , Chen W.-M. . ( 2008; ). Luteimonas aquatica sp. nov., isolated from fresh water from Southern Taiwan. . Int J Syst Evol Microbiol 58:, 2051–2055. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributed by the author.. Department of Genetics, University of Washington, Seattle, WA, USA.
  5. Finkmann W. , Altendorf K. , Stackebrandt E. , Lipski A. . ( 2000; ). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. . Int J Syst Evol Microbiol 50:, 273–282. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gomori G. . ( 1955; ). Preparation of buffers for use in enzyme studies. . Methods Enzymol 1, 138–146. [CrossRef]
    [Google Scholar]
  7. Jeon C. O. , Park W. , Ghiorse W. C. , Madsen E. L. . ( 2004; ). Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. . Int J Syst Evol Microbiol 54:, 93–97. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kahng H.-Y. , Chung B. S. , Lee D.-H. , Jung J.-S. , Park J. H. , Jeon C. O. . ( 2009; ). Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater. . Int J Syst Evol Microbiol 59:, 654–657. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kim J. M. , Le N. T. , Chung B. S. , Park J. H. , Bae J.-W. , Madsen E. L. , Jeon C. O. . ( 2008; ). Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. . Appl Environ Microbiol 74:, 7313–7320. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kim J. M. , Lee S. H. , Jung J. Y. , Jeon C. O. . ( 2010a; ). Marinobacterium lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 60:, 1828–1831. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kim J. M. , Lee H. J. , Kim S. Y. , Song J. J. , Park W. J. , Jeon C. O. . ( 2010b; ). Analysis of the fine-scale population structure of “Candidatus Accumulibacter phosphatis” in enhanced biological phosphorus removal sludge, using fluorescence in situ hybridization and flow cytometric sorting. . Appl Environ Microbiol 72:, 3825–3835. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim J. M. , Jung J. Y. , Chae H. B. , Park W. , Jeon C. O. . ( 2010c; ). Hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 60:, 2877–2881. [CrossRef] [PubMed]
    [Google Scholar]
  13. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Lee S. H. , Shim J. K. , Kim J. M. , Choi H.-K. , Jeon C. O. . ( 2011; ). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . . Int J Syst Evol Microbiol 61:, 722–727.[CrossRef]
    [Google Scholar]
  15. Lipski A. , Stackebrandt E. . ( 2005; ). Genus III. Luteimonas Finkmann, Altendorf, Stackebrandt and Lipski 2000, 280VP . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, part B, pp. 93–94. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  16. Lu S. , Park M. , Ro H.-S. , Lee D. S. , Park W. , Jeon C. O. . ( 2006; ). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. . J Microbiol 44:, 155–161.[PubMed]
    [Google Scholar]
  17. Park M. , Ryu S. H. , Vu T. H. , Ro H. S. , Yun P. Y. , Jeon C. O. . ( 2007; ). Flavobacterium defluvii sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 57:, 233–237. [CrossRef] [PubMed]
    [Google Scholar]
  18. Roh S. W. , Kim K.-H. , Nam Y.-D. , Chang H.-W. , Kim M.-S. , Yoon J.-H. , Oh H.-M. , Bae J.-W. . ( 2008; ). Luteimonas aestuarii sp. nov., isolated from tidal flat sediment. . J Microbiol 46:, 525–529. [CrossRef] [PubMed]
    [Google Scholar]
  19. Rosselló-Mora R. , Amann R. . ( 2001; ). The species concept for prokaryotes. . FEMS Microbiol Rev 25:, 39–67. [CrossRef] [PubMed]
    [Google Scholar]
  20. Sambrook J. , Russell D. W. . ( 2001; ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  21. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  22. Stamatakis A. , Ott M. , Ludwig T. . ( 2005; ). RAxML-OMP: an efficient program for phylogenetic inference on SMPs.. In Proceedings of the 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science 3506, 288–302. Springer Verlag.
    [Google Scholar]
  23. Tamaoka J. , Komagata K. . ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  24. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef] [PubMed]
    [Google Scholar]
  25. Young C.-C. , Kämpfer P. , Chen W.-M. , Yen W.-S. , Arun A. B. , Lai W.-A. , Shen F.-T. , Rekha P. D. , Lin K.-Y. , Chou J.-H. . ( 2007; ). Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste. . Int J Syst Evol Microbiol 57:, 741–744. [CrossRef] [PubMed]
    [Google Scholar]
  26. Zhang D.-C. , Liu H.-C. , Xin Y.-H. , Zhou Y.-G. , Schinner F. , Margesin R. . ( 2010; ). Luteimonas terricola sp. nov., a psychrophilic bacterium isolated from soil. . Int J Syst Evol Microbiol 60:, 1581–1584. [CrossRef] [PubMed]
    [Google Scholar]
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Transmission electron micrograph showing the general morphology of negatively stained cells of strain G3 . [PDF](215 KB). Utilization of carbon sources by strain G3 and related species. [PDF](40 KB)

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Transmission electron micrograph showing the general morphology of negatively stained cells of strain G3 . [PDF](215 KB). Utilization of carbon sources by strain G3 and related species. [PDF](40 KB)

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