1887

Abstract

During a study of the diversity and phylogeny of rhizobia in the root nodules of grown in north-western China, four strains were classified in the genus on the basis of their 16S rRNA gene sequences. The 16S rRNA gene sequences of three of these strains were identical and that of the other strain, which was the only one isolated in Yangling, differed from the others by just 1 bp. The16S rRNA gene sequences of the four strains showed a mean similarity of 99.3 % with the most closely related, recognized species, . The corresponding and gene sequences showed similarities with established species of of less than 86.5 % and less than 89.6 %, respectively. These low similarities indicated that the four strains represented a novel species of the genus . The strains were also found to be distinguishable from the closest related, established species () by rep-PCR DNA fingerprinting, analysis of cellular fatty acid profiles and from the results of a series of phenotypic tests. The level of DNA–DNA relatedness between the representative strain CCNWSX 0483 and IAM 14140 was only 40.13 %. Therefore, a novel species, sp. nov., is proposed, with strain CCNWSX 0483 ( = ACCC 14971 = HAMBI 3214) as the type strain. In nodulation and pathogenicity tests, none of the four strains of sp. nov. was able to induce any nodule or tumour formation on plants. As no amplicons were detected when DNA from the strains was run in PCR with primers for the detection of , and gene sequences, the strains probably do not carry or genes.

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2012-02-01
2019-10-24
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