1887

Abstract

Nine unidentified Gram-positive, lipophilic corynebacteria were isolated from clinical and food samples and subjected to a polyphasic taxonomic analysis. The bacteria were distinguished from species with validly published names by biochemical tests, fatty acid content and whole-cell protein analysis. Comparative 16S rRNA gene sequence analysis demonstrated unambiguously that the nine strains were related phylogenetically to the species ‘’ and represented a distinct subline within the genus . On the basis of both phenotypic and phylogenetic evidence, the formal description of sp. nov. is proposed. The type strain of is Medalle X (=LDC-20=CIP 107291=CCUG 45418=ATCC 35529).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02907-0
2004-07-01
2021-03-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/4/ijs541055.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02907-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Barreau C., Hansen W. 2000; Chimiotaxonomie. In Précis de Bactériologie Clinique , chapter 2. pp  27–52 Edited by Freney J., Renaud F., Hansen W., Bollet C. Paris: ESKA (in French;
  3. Beaman B. L., Kim K.-S., Lanéelle M. A., Barksdale L. 1974; Chemical characterization of organisms isolated from leprosy patients. J Bacteriol 117:1320–1329
    [Google Scholar]
  4. Bernard K. A., Bellefeuille M., Ewan E. P. 1991; Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J Clin Microbiol 29:83–89
    [Google Scholar]
  5. Böttger E. C. 1989; Rapid determination of bacterial ribosomal RNA sequences by direct sequencing of enzymatically amplified DNA. FEMS Microbiol Lett 65:171–176 [CrossRef]
    [Google Scholar]
  6. Brennan N. M., Brown R., Goodfellow M., Ward A. C., Beresford T. P., Simpson P. J., Fox P. F., Cogan T. M. 2001; Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51:843–852 [CrossRef]
    [Google Scholar]
  7. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  8. Brown S., Lanéelle M. A., Asselineau J., Barksdale L. 1984; Description of Corynebacterium tuberculostearicum sp.nov., a leprosy-derived Corynebacterium . Ann Microbiol (Paris) 135B:251–267
    [Google Scholar]
  9. Collins M. D., Burton R. A., Jones D. 1988; Corynebacterium amycolatum sp. nov., a new mycolic acid-less Corynebacterium species from human skin. FEMS Microbiol Lett 49:349–352 [CrossRef]
    [Google Scholar]
  10. Collins M. D., Bernard K. A., Hutson R. A., Sjödén B., Nyberg A., Falsen E. 1999a; Corynebacterium sundsvallense sp. nov., from human clinical specimens. Int J Syst Bacteriol 49:361–366 [CrossRef]
    [Google Scholar]
  11. Collins M. D., Hoyles L., Lawson P. A., Falsen E., Robson R. L., Foster G. 1999b; Phenotypic and phylogenetic characterization of a new Corynebacterium species from dogs: description of Corynebacterium auriscanis sp. nov. J Clin Microbiol 37:3443–3447
    [Google Scholar]
  12. Collins M. D., Hoyles L., Foster G., Sjödén B., Falsen E. 2001a; Corynebacterium capitovis sp. nov., from a sheep. Int J Syst Evol Microbiol 51:857–860 [CrossRef]
    [Google Scholar]
  13. Collins M. D., Hoyles L., Hutson R. A., Foster G., Falsen E. 2001b; Corynebacterium testudinoris sp. nov. from a tortoise, and Corynebacterium felinum sp. nov., from a Scottish wild cat. Int J Syst Evol Microbiol 51:1349–1352
    [Google Scholar]
  14. Danhaive P., Hoet P., Cocito C. 1982; Base compositions and homologies of deoxyribonucleic acids of corynebacteria isolated from human leprosy lesions and of related microorganisms. Int J Syst Bacteriol 32:70–76 [CrossRef]
    [Google Scholar]
  15. Drancourt M., Bollet C., Carlioz A., Martelin R., Gayral J. P., Raoult D. 2000; 16S ribosomal DNA sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. J Clin Microbiol 38:3623–3630
    [Google Scholar]
  16. Esteban J., Nieto E., Calvo R., Fernandez-Roblas R., Valero-Guillen P. L., Soriano F. 1999; Microbial characterization and clinical significance of Corynebacterium amycolatum strains. Eur J Clin Microbiol Infect Dis 18:518–521 [CrossRef]
    [Google Scholar]
  17. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  18. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  19. Felsenstein J. 1993 phylip: phylogenetic inference package, version 3.53c. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  20. Fernandez-Garayzabal J. F., Collins M. D., Hutson R. A., Fernandez E., Monasterio R., Marco J., Dominguez L. 1997; Corynebacterium mastitidis sp. nov., isolated from milk of sheep with subclinical mastitis. Int J Syst Bacteriol 47:1082–1085 [CrossRef]
    [Google Scholar]
  21. Fudou R., Jojima Y., Seto A., Yamada K., Kimura E., Nakamatsu T., Hiraishi A., Yamanaka S. 2002; Corynebacterium efficiens sp. nov., a glutamic-acid-producing species from soil and vegetables. Int J Syst Evol Microbiol 52:1127–1131 [CrossRef]
    [Google Scholar]
  22. Funke G., Bernard K. A., Bucher C., Pfyffer G. E., Collins M. D. 1995; Corynebacterium glucuronolyticum sp. nov. isolated from male patients with genitourinary infections. Med Microbiol Lett 4:204–215
    [Google Scholar]
  23. Funke G., von Graevenitz A., Clarridge J. E. III, Bernard K. A. 1997a; Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10:125–159
    [Google Scholar]
  24. Funke G., Hutson R. A., Hilleringmann M., Heizmann W. R., Collins D. 1997b; Corynebacterium lipophiloflavum sp. nov. isolated from a patient with bacterial vaginosis. FEMS Microbiol Lett 150:219–224 [CrossRef]
    [Google Scholar]
  25. Funke G., Lawson P. A., Collins M. D. 1997c; Corynebacterium mucifaciens sp. nov., an unusual species from human clinical material. Int J Syst Bacteriol 47:952–957 [CrossRef]
    [Google Scholar]
  26. Funke G., Osorio C. R., Frei R., Riegel P., Collins M. D. 1998; Corynebacterium confusum sp. nov., isolated from human clinical specimens. Int J Syst Bacteriol 48:1291–1296 [CrossRef]
    [Google Scholar]
  27. Furness G., Sambury S., Evangelista A. T. 1979; Corynebacterium pseudogenitalium sp. nov. Commensals of the human male and female urogenital tracts. Invest Urol 16:292–295
    [Google Scholar]
  28. Goyache J., Vela A. I., Collins M. D. 7 other authors 2003; Corynebacterium spheniscorum sp. nov., isolated from the cloacae of wild penguins. Int J Syst Evol Microbiol 53:43–46 [CrossRef]
    [Google Scholar]
  29. Higgins D. G. 1994; clustal v: multiple alignment of DNA and protein sequences. Methods Mol Biol 25:307–318
    [Google Scholar]
  30. Hollis D. G., Weaver R. E. 1981 Gram-positive Organisms: a Guide to Identification Atlanta, GA: Special Bacteriology Section, Centers for Disease Control;
    [Google Scholar]
  31. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–223 [CrossRef]
    [Google Scholar]
  32. Neubauer M., Sourek J., Ryc M., Bohacek J., Mara M., Mnukova J. 1991; Corynebacterium accolens sp. nov., a gram-positive rod exhibiting satellitism, from clinical material. Syst Appl Microbiol 14:46–51 [CrossRef]
    [Google Scholar]
  33. Pascual C., Lawson P. A., Farrow J. A. E., Gimenez M. N., Collins M. D. 1995; Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences. Int J Syst Bacteriol 45:724–728 [CrossRef]
    [Google Scholar]
  34. Pascual C., Foster G., Alvarez N., Collins M. D. 1998; Corynebacterium phocae sp. nov., isolated from the common seal ( Phoca vitulina . Int J Syst Bacteriol 48:601–604 [CrossRef]
    [Google Scholar]
  35. Paviour S., Musaad S., Roberts S., Taylor G., Taylor S., Shore K., Lang S., Holland D. 2002; Corynebacterium species isolated from patients with mastitis. Clin Infect Dis 35:1434–1440 [CrossRef]
    [Google Scholar]
  36. Phetsuksiri B., Baulard A. R., Cooper A., Minnikin D. E., Douglas J. D., Besra G. S., Brennan P. J. 1999; Antimycobacterial activities of isoxyl and new derivatives through the inhibition of mycolic acid synthesis. Antimicrob Agents Chemother 43:1042–1051
    [Google Scholar]
  37. Renaud F. N. R., Aubel D., Riegel P., Meugnier H., Bollet C. 2001; Corynebacterium freneyi sp. nov., α -glucosidase-positive strains related to Corynebacterium xerosis . Int J Syst Evol Microbiol 51:1723–1728 [CrossRef]
    [Google Scholar]
  38. Riegel P., Grimont P. A. D., De Briel D., Ageron E., Jehl F., Pelegrin M., Monteil H., Monck R. 1992; Corynebacterium group D2 (‘ Corynebacterium urealyticum ’) constitutes a new genomic species. Res Microbiol 143:307–313 [CrossRef]
    [Google Scholar]
  39. Riegel P., De Briel D., Prevost G., Jehl F., Monteil H., Minck R. 1993; Taxonomic study of Corynebacterium group ANF-1 strains: proposal of Corynebacterium afermentans sp. nov. containing the subspecies C.afermentans subsp. afermentans subsp.nov. and C.afermentans subsp. lipophilum subsp. nov. Int J Syst Bacteriol 43:287–292 [CrossRef]
    [Google Scholar]
  40. Riegel P., Ruimy R., De Briel D., Prévost G., Jehl F., Christen R., Monteil H. 1995a; Genomic diversity and phylogenetic relationships among lipid-requiring diphtheroids from humans and characterization of Corynebacterium macginleyi sp. nov. Int J Syst Bacteriol 45:128–133 [CrossRef]
    [Google Scholar]
  41. Riegel P., Ruimy R., De Briel D., Prevost G., Jehl F., Bimet F., Christen R., Monteil H. 1995b; Corynebacterium argentoratense sp. nov., from the human throat. Int J Syst Bacteriol 45:533–537 [CrossRef]
    [Google Scholar]
  42. Riegel P., Ruimy R., Renaud F. N. R., Freney J., Prevost G., Jehl F., Christen R., Monteil H. 1997a; Corynebacterium singulare sp. nov., a new species for urease-positive strains related to Corynebacterium minutissimum . Int J Syst Bacteriol 47:1092–1096 [CrossRef]
    [Google Scholar]
  43. Riegel P., Heller R., Prevost G., Jehl F., Monteil H. 1997b; Corynebacterium durum sp. nov., from human clinical specimens. Int J Syst Bacteriol 47:1107–1111 [CrossRef]
    [Google Scholar]
  44. Ruimy R., Riegel P., Boiron P., Monteil H., Christen R. 1995; Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences. Int J Syst Bacteriol 45:740–746 [CrossRef]
    [Google Scholar]
  45. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  46. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  47. Swofford D. L. 1998 paup*. Phylogenetic analysis using parsimony (*and other methods), version 4 Sunderland, MA: Sinauer;
    [Google Scholar]
  48. Tamura K., Nei M. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526
    [Google Scholar]
  49. Watts J. L., Lowery D. E., Teel J. F., Rossbach S. 2000; Identification of Corynebacterium bovis and other coryneforms isolated from bovine mammary glands. J Dairy Sci 83:2373–2379 [CrossRef]
    [Google Scholar]
  50. Yassin A. F., Steiner U., Ludwig W. 2002a; Corynebacterium aurimucosum sp. nov. and emended description of Corynebacterium minutissimum Collins and Jones (1983). Int J Syst Evol Microbiol 52:1001–1005 [CrossRef]
    [Google Scholar]
  51. Yassin A. F., Steiner U., Ludwig W. 2002b; Corynebacterium appendicis sp. nov. Int J Syst Evol Microbiol 52:1165–1169 [CrossRef]
    [Google Scholar]
  52. Zimmermann O., Spröer C., Kroppenstedt R. M., Fuchs E., Köchel H. G., Funke G. 1998; Corynebacterium thomssenii sp. nov., a Corynebacterium with N -acetyl- β -glucosaminidase activity from human clinical specimens. Int J Syst Bacteriol 48:489–494 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02907-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02907-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error