1887

Abstract

An analysis of 16S rRNA gene sequences from archived clinical reference specimens identified a novel species of the genus , of which four strains have been independently isolated from human blood. On the basis of 16S rRNA gene sequence similarity, the closest relatives with validly published names were R7 (98.7 %), CECT 5989 (97.7 %), Iso-46 (97.6 %) and IMMIB L-1110 (97.2 %) Maximum-likelihood phylogenetic analysis of 16S rRNA gene sequences showed that the isolates belonged to the genus and were members of a cluster associated with sp. PRwf-1, isolated from a silk snapper fish. DNA–DNA relatedness and partial 23S rRNA gene sequences also supported the finding that the isolates belonged to a species distinct from its closest phylogenetic neighbours. The predominant cellular fatty acids were Cω9, C, summed feature 3 (Cω7 and/or iso-C 2-OH), summed feature 5 (Cω6,9 and/or anteiso-C) and C. Biochemical and morphological analysis further supported the assignment of the four isolates to a novel species. The name sp. nov. is proposed. The type strain is 13983 ( = DSM 23635 = CCUG 59771).

Funding
This study was supported by the:
  • NASA Astrobiology Institute (Award NCC2-1274)
  • CDC
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2012-01-01
2024-04-19
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Anisimova M., Gascuel O. 2006; Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. Syst Biol 55:539–552 [View Article][PubMed]
    [Google Scholar]
  3. Bowman J. P. 2006; The genus Psychrobacter . In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn. vol. 3 pp. 920–930 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  4. Bowman J. P., Cavanagh J., Austin J. J., Sanderson K. 1996; Novel Psychrobacter species from Antarctic ornithogenic soils. Int J Syst Bacteriol 46:841–848 [View Article][PubMed]
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  6. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [View Article][PubMed]
    [Google Scholar]
  7. Kämpfer P., Albrecht A., Buczolits S., Busse H.-J. 2002; Psychrobacter faecalis sp. nov., a new species from a bioaerosol originating from pigeon faeces. Syst Appl Microbiol 25:31–36 [View Article][PubMed]
    [Google Scholar]
  8. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  9. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . Nucleic Acids Res 35:7188–7196 [View Article][PubMed]
    [Google Scholar]
  10. Romanenko L. A., Lysenko A. M., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54:1741–1745 [View Article][PubMed]
    [Google Scholar]
  11. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V. 2009; Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan. Int J Syst Evol Microbiol 59:1480–1486 [View Article][PubMed]
    [Google Scholar]
  12. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  13. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  14. Vela A. I., Collins M. D., Latre M. V., Mateos A., Moreno M. A., Hutson R., Domínguez L., Fernández-Garayzábal J. F. 2003; Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int J Syst Evol Microbiol 53:415–419 [View Article][PubMed]
    [Google Scholar]
  15. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  16. Wolfgang W. J., Carpenter A. N., Cole J. A., Gronow S., Habura A., Jose S., Nazarian E. J., Kohlerschmidt D. J., Limberger R. et al. other authors 2011; Neisseria wadsworthii sp. nov. and Neisseria shayeganii sp. nov. isolated from clinical specimens. Int J Syst Evol Microbiol 61:91–98[PubMed] [CrossRef]
    [Google Scholar]
  17. Yassin A. F., Busse H.-J. 2009; Psychrobacter lutiphocae sp. nov., isolated from the faeces of a seal. Int J Syst Evol Microbiol 59:2049–2053 [View Article][PubMed]
    [Google Scholar]
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