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Abstract

A strict anaerobe (strain GB8-1) that degraded straight-chain fatty acids with C–C in syntrophic association with methanogens was isolated from an up-flow anaerobic sludge blanket reactor treating beer wastewater. Strain GB8-1 degraded 1 mol butyrate into about 2 mol acetate and 1 mol (presumably) H in co-culture with a methanogen. Neither branched-chain fatty acids nor benzoate could be degraded. Strain GB8-1 could grow on crotonate in pure culture and converted 1 mol crotonate to 0·5 mol butyrate and 1 mol acetate. Generation time was about 11 h when grown on crotonate at 37 °C. Fumarate, sulfate, thiosulfate, sulfur and nitrate could not serve as electron acceptors for strain GB8-1 to degrade butyrate. Cells of strain GB8-1 were curved rods with Gram-negative cell walls; no spores were observed. The DNA G+C content was 46·6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain GB8-1 was related most closely to the fatty acid-oxidizing, syntrophic bacterium S DSM 3441; however, the relationship was not very close (95·4 % sequence similarity). Some phenotypic features also differentiated strain GB8-1 from DSM 3441. Therefore, a novel species, sp. nov., is proposed. The type strain is GB8-1 (=CGMCC 1.5010=DSM 15682).

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2004-05-01
2021-04-19
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