1887

Abstract

A novel actinomycete strain, YIM 001, was isolated from a soil sample collected from Hebei province, People's Republic of China. The strain was characterized by white to grey aerial mycelium. Long spore chains, borne on the aerial mycelium, were straight to ; the spore chains were composed of non-motile and coccoid spores with a warty surface. The cell wall of strain YIM 001 contained -diaminopimelic acid (Apm) and traces of -Apm. Whole-cell hydrolysates contained mainly glucose and small amounts of xylose, galactose and arabinose. The menaquinones were MK-9(H) (4·6 %), MK-9(H) (60 %), MK-9(H) (30·7 %) and MK-9(H) (4·7 %). Phosphatidylethanolamine was the diagnostic phospholipid. The DNA G+C content of strain YIM 001 was 71·4 mol%. Phylogenetic analysis indicated that strain YIM 001 belongs to the genus . Based on its phenotypic and genotypic characteristics, strain YIM 001 (=CCTCC AA 203005=CIP 107974=DSM 41837) is proposed as the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02897-0
2004-05-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/54/3/ijs540727.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02897-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparation from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243
    [Google Scholar]
  3. Bérdy J. 1995; Are actinomycetes exhausted as a source of secondary metabolites?. Biotechnologia 7:813–34
    [Google Scholar]
  4. Chun J., Youn H.-D., Yim Y.-I., Lee H., Kim M. Y., Hah Y. C., Kang S.-O. 1997; Streptomyces seoulensis sp. nov. Int J Syst Bacteriol 47:492–498 [CrossRef]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  6. Cui X.-L., Mao P.-H., Zeng M., Li W.-J., Zhang L.-P., Xu L.-H., Jiang C.-L. 2001; Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . Int J Syst Evol Microbiol 51:357–363
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  10. Hayakawa M., Nonomura H. 1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [CrossRef]
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  12. Jiang L. Y., Li M. G., Li W. J., Cui X. L., Xu L. H., Jiang C. L. 2001; Study on the application of quantitative analysis of cell-wall amino acids in actinomycetes. Acta Microbiol Sin 41:270–277
    [Google Scholar]
  13. Kelly K. L. 1964 Color-name Charts Illustrated with Centroid Colors Chicago: Inter-Society Color Council-National Bureau of Standards;
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  15. Labeda D. P., Lechevalier M. P., Testa R. T. 1997; Streptomyces stramineus sp. nov., a new species of the verticillate streptomycetes. Int J Syst Bacteriol 47:747–753 [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy pp  227–291 Edited by Dietz A., Thayer D. W. Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  17. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentbl Bakteriol Hyg 1 Abt Suppl 11:111–116
    [Google Scholar]
  18. Li W.-J., Zhang L.-P., Xu P., Cui X.-L., Lu Z.-T., Xu L.-H., Jiang C.-L. 2002; Streptomyces beijiangensis sp. nov., a psychrotolerant actinomycete isolated from soil in China. Int J Syst Evol Microbiol 52:1695–1699 [CrossRef]
    [Google Scholar]
  19. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  22. Shirling E. B., Gottlieb D. 1968; Cooperative description of type cultures of Streptomyces . II. Species descriptions from first study. Int J Syst Bacteriol 18:69–189 [CrossRef]
    [Google Scholar]
  23. Shirling E. B., Gottlieb D. 1972; Cooperative description of type cultures of Streptomyces . V. Additional descriptions. Int J Syst Bacteriol 22:265–394 [CrossRef]
    [Google Scholar]
  24. Takeuchi M., Hatano K. 1998; Gordonia rhizosphera sp. nov. isolated from the mangrove rhizosphere. Int J Syst Bacteriol 48:907–912 [CrossRef]
    [Google Scholar]
  25. Tatusova T. A., Madden T. L. 1999; blast 2 Sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174:247–250 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  27. Waksman S. A., Henrici A. 1943; The nomenclature and classification of the actinomycetes. J Bacteriol 46:337–341
    [Google Scholar]
  28. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  29. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2452–2492 Edited by Williams S. T., Sharpe M. E, Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.02897-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02897-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error