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Abstract

A Gram-positive, strictly aerobic, rod-shaped, non-motile bacterial strain, designated MJ28, was isolated from a sludge sample from the Daejeon sewage disposal plant in South Korea. A polyphasic approach was applied to study the taxonomic position of strain MJ28. Strain MJ28 showed highest 16S rRNA gene sequence similarity to KP02 (95.2 %). Levels of 16S rRNA gene sequence similarity to the type strains of other species were less than 94.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ28 belonged to the clade formed by members of the genus in the family . The G+C content of the genomic DNA of strain MJ28 was 65.8 mol%. The chemotaxonomic characteristics of strain MJ28 showed features typical of the genus , with MK-9 as the predominant respiratory quinone, 2,4-diaminobutryic acid as the diamino acid in the peptidoglycan, and anteiso-C (44.6 %), anteiso-C (35.7 %) and C (9.5 %) as the major fatty acids. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, strain MJ28 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ28 ( = KCTC 19773 = JCM 16921).

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2012-04-01
2019-12-06
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