1887

Abstract

A reddish-brown bacterium was isolated from photoheterotrophic enrichments of mangrove soil from the western coast of India, in a medium that contained 10 % (w/v) NaCl. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA100 clusters with species of the genus of the class ‘’. Cells of strain JA100 are Gram-negative, motile rods with monopolar single flagella; they require NaCl, the optimum concentration being 1–4 %, and tolerate concentrations up to 13 %. The strain has vesicular internal membrane structures, bacteriochlorophyll and, most probably, carotenoids of the spirilloxanthin series. No growth factors are required. A reduced sulfur source is required for growth, and, during growth on reduced sulfur sources as electron donors, sulfur is intermediately deposited as a single large granule within the cell. Strain JA100 could not grow at the expense of other tricarboxylic acid cycle intermediates, except malate. On the basis of 16S rRNA gene sequence analysis and its morphological and physiological characteristics, strain JA100 is sufficiently different from other species to justify its designation as a novel species, for which the name sp. nov. is proposed. The type strain is JA100 (=DSM 15907=ATCC BAA-741=JCM 12653).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02892-0
2005-03-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/55/2/ijs550673.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02892-0&mimeType=html&fmt=ahah

References

  1. Bast, E. ( 1986; ). Urease formation in purple sulfur bacteria (Chromatiaceae) grown on various nitrogen sources. Arch Microbiol 146, 199–203.[CrossRef]
    [Google Scholar]
  2. Biebl, H. & Pfennig, N. ( 1981; ). Isolation of members of the family Rhodospirillaceae. In The Prokaryotes, pp. 167–273. Edited by M. P. Starr, H. Stolp, H. G. Truper, A. Balows & H. G. Schlegel. New York: Springer.
  3. Bryantseva, I. A., Gorlenko, V. M., Kompantseva, E. I. & Imhoff, J. F. ( 2000; ). Thioalkalicoccus limnaeus gen. nov., sp. nov., a new alkaliphilic purple sulfur bacterium with bacteriochlorophyll b. Int J Syst Evol Microbiol 50, 2157–2163.[CrossRef]
    [Google Scholar]
  4. Burgess, J. G., Kawaguchi, R., Yamada, A. & Matsunaga, T. ( 1994; ). Rhodobacter marinus sp. nov.: a new marine hydrogen producing photosynthetic bacterium which is sensitive to oxygen and sulphide. Microbiology 140, 965–970.[CrossRef]
    [Google Scholar]
  5. Caumette, P., Imhoff, J. F., Suling, J. & Matheron, R. ( 1997; ). Chromatium glycolicum sp. nov., a moderately halophilic purple sulfur bacterium that uses glycolate as substrate. Arch Microbiol 167, 11–18.[CrossRef]
    [Google Scholar]
  6. Eimhjellen, K. E. ( 1970; ). Thiocapsa pfennigii sp. nov. a new species of the phototrophic sulfur bacteria. Arch Microbiol 73, 193–194.
    [Google Scholar]
  7. Felsenstein, J. ( 1989; ). phylip – phylogeny inference package (version 3.2). Cladistics 5, 164–166.
    [Google Scholar]
  8. Guyoneaud, R., Süling, J., Petri, R., Matheron, R., Caumette, P., Pfennig, N. & Imhoff, J. F. ( 1998; ). Taxonomic rearrangements of the genera Thiocapsa and Amoebobacter on the basis of 16S rDNA sequence analyses, and description of Thiolamprovum gen. nov. Int J Syst Bacteriol 48, 957–964.[CrossRef]
    [Google Scholar]
  9. Imhoff, J. F. ( 1995; ). Taxonomy and physiology of phototrophic purple bacteria and green sulfur bacteria. In Anoxygenic Photosynthetic Bacteria, pp. 1–15. Edited by R. E. Blankenship, M. T. Madigan & C. E. Bauer. The Netherlands: Kluwer.
  10. Imhoff, J. F. ( 2001a; ). The anoxygenic phototrophic purple bacteria. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 631–637. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  11. Imhoff, J. F. ( 2001b; ). True marine and halophilic anoxygenic phototrophic bacteria. Arch Microbiol 176, 243–254.[CrossRef]
    [Google Scholar]
  12. Imhoff, J. F. & Pfennig, N. ( 2001; ). Thioflavicoccus mobilis gen. nov., sp. nov., a novel purple sulfur bacterium with bacteriochlorophyll b. Int J Syst Evol Microbiol 51, 105–110.
    [Google Scholar]
  13. Imhoff, J. F. & Süling, J. ( 1996; ). The phylogenetic relationship among Ectothiorhodospiraceae: a reevaluation of their taxonomy on the basis of 16S rDNA analysis. Arch Microbiol 165, 106–113.[CrossRef]
    [Google Scholar]
  14. Imhoff, J., Süling, J. & Petri, R. ( 1998; ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium. Int J Syst Bacteriol 48, 1129–1143.[CrossRef]
    [Google Scholar]
  15. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  16. Madigan, M. T. ( 2001; ). Family VI. “Heliobacteriaceae” Beer-Romero and Gest 1987, 113. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 625–630. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  17. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  18. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  19. Pfennig, N. & Trüper, H. G. ( 1989; ). I. Purple bacteria. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 1637–1653. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  20. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w : improving the sensitivity of progressive multiple sequence alignment though sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  21. Trüper, H. G. & Pfennig, N. ( 1981; ). Characterization and identification of the anoxygenic phototrophic bacteria. In The Prokaryotes, pp. 299–312. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. New York: Springer.
  22. Van Gemerden, H. & Mas, J. ( 1995; ). Ecology of phototrophic sulfur bacteria. In Anoxygenic Photosynthetic Bacteria, pp. 49–85. Edited by R. E. Blankenship, M. T. Madigan & C. E. Bauer. The Netherlands: Kluwer.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02892-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02892-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error