1887

Abstract

Two strains, designated B1-1 and B6-8, were isolated from the Geumho River and the Dalseo Stream in Korea. Comparative 16S rRNA gene sequence analysis showed a clear affiliation of these two bacteria with the class , their closest relatives being KCTC 12095, KCTC 12575, KACC 12605 and KACC 12910 with 16S rRNA gene sequence similarities of 95.3 –97.7 % to the two novel strains. Strains B1-1 and B6-8 shared a 16S rRNA gene sequence similarity value of 96.1 %. Cells of the two strains were Gram-reaction-negative, aerobic, non-motile, short rods or cocci. The predominant ubiquinone was Q-10. The major fatty acids were C Cω7, C and Cω8 cyclo for strain B1-1 and C Cω7, C, C 2-OH, and Cω8 cyclo for strain B6-8. The G+C contents of the genomic DNA of the strains B1-1 and B6-8 were 61.6 and 66.5 mol%, respectively. Based on the results of this polyphasic study, strains B1-1 ( = KCTC 12849  = DSM 18799) and B6-8 ( = KCTC 12850  = DSM 18800) represent two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed, respectively.

Funding
This study was supported by the:
  • , 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology , (Award MG08-0101-2-0)
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2011-11-01
2020-08-11
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