1887

Abstract

A novel extremely thermophilic, methane-producing archaeon was isolated from a black smoker chimney at the Kairei field in the Central Indian Ridge. Cells of this isolate were irregular cocci with several flagella; motility was not observed. Growth was observed between 55 and 83 °C (optimum of 75 °C; 30 min doubling time) and between pH 6·0 and 8·5 (optimum of pH 6·7). The isolate was a strictly anaerobic, methanogenic autotroph capable of using hydrogen and carbon dioxide as sole energy and carbon sources. Formate was utilized as an alternative energy source. The G+C content of the genomic DNA was 33·3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was most closely related to strain Kol 5. The isolate, however, could be genetically differentiated from this species by DNA–DNA hybridization analysis and on the basis of its physiological properties. The name sp. nov. is proposed for this isolate; the type strain is Mc-S-70 (=JCM 11930=ATCC BAA-687).

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2004-07-01
2019-10-24
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