1887

Abstract

Four arthroconidium-producing yeasts were isolated from the gut of wood-inhabiting tenebrionid and passalid beetles. The rRNA genes of these yeast strains were sequenced, compared and analysed. The sequence results and other taxonomic characterizations placed two of the strains into , and the remaining strains, EH024 and EH026 which were isolated from (Coleoptera: Tenebrionidae), into a novel species of the genus in the Porosum clade. Strain EN6S23 was independently isolated from forest soil in Taiwan and was identified as the same novel species based on identical sequences in the internal transcribed spacers (ITS) and the D1/D2 region of the LSU rRNA gene and similar physiological characteristics to those of strains EH024 and EH026. The three strains can assimilate cellulose and xylan as sole carbon source, and are clearly distinguished from their closest taxon, , by 14 nt differences in the ITS and D1/D2 region. These strains did not reproduce sexually under the laboratory conditions tested. The novel species is proposed as sp. nov. (type strain EH024  = ATCC MYA-4670  = CBS 11841).

Funding
This study was supported by the:
  • National Science Foundation (Award DBI-0548684)
  • ATCC Internship Program
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2011-10-01
2024-04-20
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Barnett J. A., Payne R. W., Yarrow D. 2000 Yeasts: Characteristics and Identification, 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Breznak J. A., Brune A. 1994; Role of microorganisms in the digestion of lignocellulose by termites. Annu Rev Entomol 39:453–487 [View Article]
    [Google Scholar]
  4. Cline A. R., Leschen R. A. B. 2005; Coleoptera associated with the oyster mushroom, Pleurotus ostreatus Fries, in North America. Southeast Nat 4:409–420 [View Article]
    [Google Scholar]
  5. Fell J. W., Scorzetti G. 2004; Reassignment of the basidiomycetous yeasts Trichosporon pullulans to Guehomyces pullulans gen. nov., comb. nov. and Hyalodendron lignicola to Trichosporon lignicola comb. nov.. Int J Syst Evol Microbiol 54:995–998 [View Article][PubMed]
    [Google Scholar]
  6. Fell J. W., Boekhout T., Fonseca A., Scorzetti G., Statzell-Tallman A. 2000; Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50:1351–1371 [View Article][PubMed]
    [Google Scholar]
  7. Fuentefria A. M., Suh S.-O., Landell M. F., Faganello J., Schrank A., Vainstein M. H., Blackwell M., Valente P. 2008; Trichosporon insectorum sp. nov., a new anamorphic basidiomycetous killer yeast. Mycol Res 112:93–99 [View Article][PubMed]
    [Google Scholar]
  8. Guého E., Smith M. Th., de Hoog G. S. 1998; Trichosporon Behrend. In The Yeasts, a Taxonomic Study, 4th edn. pp. 854–872 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
  9. Kulakovskaya T. V., Golubev W. I., Tomashevskaya M. A., Kulakovskaya E. V., Shashkov A. S., Grachev A. A., Chizhov A. S., Nifantiev N. E. 2010; Production of antifungal cellobiose lipids by Trichosporon porosum . Mycopathologia 169:117–123 [View Article][PubMed]
    [Google Scholar]
  10. Lee C. F., Yao C. H., Liu Y. R., Young S. S., Chang K. S. 2009; Kazachstania wufongensis sp. nov., an ascosporogenous yeast isolated from soil in Taiwan. Antonie van Leeuwenhoek 95:335–341 [View Article][PubMed]
    [Google Scholar]
  11. Liu C. H., Young S. S., Chang T. C., Lee C. F. 2008; Candida dajiaensis sp. nov., Candida yuanshanicus sp. nov., Candida jianshihensis sp. nov., and Candida sanyiensis sp. nov., four anamorphic, ascomycetous yeast species isolated from soil in Taiwan. FEMS Yeast Res 8:815–822 [View Article][PubMed]
    [Google Scholar]
  12. Majka C. G., Bouchard P., Bousquet Y. 2008; Tenebrionidae (Coleoptera) of the Maritime Provinces of Canada. Can Entomol 140:690–713 [View Article]
    [Google Scholar]
  13. Middelhoven W. J. 2004; Trichosporon wieringae sp. nov., an anamorphic basidiomycetous yeast from soil, and assimilation of some phenolic compounds, polysaccharides and other non-conventional carbon sources by saprophytic Trichosporon species. Antonie van Leeuwenhoek 86:329–337 [View Article][PubMed]
    [Google Scholar]
  14. Middelhoven W. J., Scorzetti G., Fell J. W. 2001; Trichosporon porosum comb. nov., an anamorphic basidiomycetous yeast inhabiting soil, related to the loubieri/laibachii group of species that assimilate hemicelluloses and phenolic compounds. FEMS Yeast Res 1:15–22[PubMed]
    [Google Scholar]
  15. Middelhoven W. J., Scorzetti G., Fell J. W. 2004; Systematics of the anamorphic basidiomycetous yeast genus Trichosporon Behrend with the description of five novel species: Trichosporon vadense, T. smithiae, T. dehoogii, T. scarabaeorum and T. gamsii . Int J Syst Evol Microbiol 54:975–986 [View Article][PubMed]
    [Google Scholar]
  16. Minch E. L. 1952; Insect inhabitants of Polyporus betulinus . J NY Entomol Soc 60:31–35
    [Google Scholar]
  17. Molnar O., Schatzmayr G., Fuchs E., Prillinger H. 2004; Trichosporon mycotoxinivorans sp. nov., a new yeast species useful in biological detoxification of various mycotoxins. Syst Appl Microbiol 27:661–671 [View Article][PubMed]
    [Google Scholar]
  18. Okoli I., Oyeka C. A., Kwon-Chung K. J., Theelen B., Robert V., Groenewald J. Z., McFadden D. C., Casadevall A., Boekhout T. 2007; Cryptotrichosporon anacardii gen. nov., sp. nov., a new trichosporonoid capsulate basidiomycetous yeast from Nigeria that is able to form melanin on niger seed agar. FEMS Yeast Res 7:339–350 [View Article][PubMed]
    [Google Scholar]
  19. Pointing S. B. 1999; Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi. Fungal Divers 2:17–33
    [Google Scholar]
  20. Puchkov E. O., Zähringer U., Lindner B., Kulakovskaya T. V., Seydel U., Wiese A. 2002; The mycocidal, membrane-active complex of Cryptococcus humicola is a new type of cellobiose lipid with detergent features. Biochim Biophys Acta 1558:161–170 [View Article][PubMed]
    [Google Scholar]
  21. Robinson R. 1918; Beetles collected on a dead black oak in Virginia. J NY Entomol Soc 26:30–33
    [Google Scholar]
  22. Schäfer A., Konrad R., Kuhnigk T., Kämpfer P., Hertel H., König H. 1996; Hemicellulose-degrading bacteria and yeasts from the termite gut. J Appl Bacteriol 80:471–478[PubMed] [CrossRef]
    [Google Scholar]
  23. Scorzetti G., Fell J. W., Fonseca A., Statzell-Tallman A. 2002; Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517[PubMed] [CrossRef]
    [Google Scholar]
  24. Sugita T., Nishikawa A., Ikeda R., Shinoda T. 1999; Identification of medically relevant Trichosporon species based on sequences of internal transcribed spacer regions and construction of a database for Trichosporon identification. J Clin Microbiol 37:1985–1993[PubMed]
    [Google Scholar]
  25. Sugita T., Takashima M., Nakase T., Ichikawa T., Ikeda R., Shinoda T. 2001; Two new yeasts, Trichosporon debeurmannianum sp. nov. and Trichosporon dermatis sp. nov., transferred from the Cryptococcus humicola complex. Int J Syst Evol Microbiol 51:1221–1228 [View Article][PubMed]
    [Google Scholar]
  26. Sugita T., Kikuchi K., Makimura K., Urata K., Someya T., Kamei K., Niimi M., Uehara Y. 2005; Trichosporon species isolated from guano samples obtained from bat-inhabited caves in Japan. Appl Environ Microbiol 71:7626–7629 [View Article][PubMed]
    [Google Scholar]
  27. Suh S.-O., Blackwell M. 2004; Three new beetle-associated yeasts in the Pichia guilliermondii clade. FEMS Yeast Res 5:87–95 [View Article]
    [Google Scholar]
  28. Suh S.-O., Blackwell M. 2005; Four new yeasts in the Candida mesenterica clade associated with basidiocarp-feeding beetles. Mycologia 97:167–177 [View Article][PubMed]
    [Google Scholar]
  29. Suh S.-O., Zhou J. J. 2010; Methylotrophic yeasts near Ogataea (Hansenula) polymorpha: a proposal of O. angusta comb. nov. and Candida parapolymorpha sp. nov. FEMS Yeast Res 10:631–638 [View Article]
    [Google Scholar]
  30. Suh S.-O., McHugh J. V., Blackwell M. 2004; Expansion of the Candida tanzawaensis yeast clade: 16 novel Candida species from basidiocarp-feeding beetles. Int J Syst Evol Microbiol 54:2409–2429 [View Article][PubMed]
    [Google Scholar]
  31. Suh S.-O., McHugh J. V., Pollock D. D., Blackwell M. 2005; The beetle gut: a hyperdiverse source of novel yeasts. Mycol Res 109:261–265 [View Article][PubMed]
    [Google Scholar]
  32. Swofford D. L. 2002; paup*: Phylogenetic analysis using parsimony (and other methods), version 4.0b10. Sunderland, MA: Sinauer Associates;
  33. Tatusova T. A., Madden T. L. 1999; blast 2 sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174:247–250 [View Article][PubMed]
    [Google Scholar]
  34. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  35. Varma A., Kolli B. K., Paul J., Saxena S., König H. 1994; Lignocellulose degradation by microorganisms from termite hills and termite guts: A survey on the present state of art. FEMS Microbiol Rev 15:9–28 [View Article]
    [Google Scholar]
  36. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp. 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier; [View Article]
    [Google Scholar]
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