1887

International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 10

gen. nov., sp. nov., a slightly thermophilic bacterium of the phylum ‘’ and the proposal of fam. nov. Free

Abstract

A bacterial isolate, with an optimum growth temperature of about 50 °C and an optimum pH for growth between 7.5 and 8.5, was recovered from a hot spring in the Furnas area of the Island of São Miguel in the Azores. The novel isolate is orange-pigmented, forms non-motile, rod-shaped cells that stain Gram-negative, is strictly aerobic, oxidase-positive and catalase-negative. The major fatty acids of strain TU-20 are 16 : 1ω6 17 : 0 iso 3-OH and 15 : 0 iso. Menaquinone 6 is the major respiratory quinone. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics, we describe a novel species of a novel genus represented by strain TU-20 ( = DSM 21410 = LMG 24594) for which we propose the name gen nov., sp. nov. We also propose the family fam. nov. to accommodate this new genus.

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2011-10-01
2020-12-04
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References

  1. Albuquerque L., Simões C., Nobre M. F., Pino N. M., Battista J. R., Silva M. T., Rainey F. A., da Costa M. S. 2005; Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov.. FEMS Microbiol Lett 247:161–169 [CrossRef][PubMed]
     [Google Scholar]
  2. Albuquerque L., Rainey F. A., Nobre M. F., da Costa M. S. 2008; Elioraea tepidiphila gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria . Int J Syst Evol Microbiol 58:773–778 [CrossRef][PubMed]
     [Google Scholar]
  3. Albuquerque L., Rainey F. A., Nobre M. F., da Costa M. S. 2010; Meiothermus granaticius sp. nov., a new slightly thermophilic red-pigmented species from the Azores. Syst Appl Microbiol 33:243–246 [CrossRef][PubMed]
     [Google Scholar]
  4. Alfredsson G. A., Kristjansson J. K., Hjörleifsdottir S. H., Stetter K. O. 1988; Rhodothermus marinus, gen. nov., sp. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol 134:299–306
     [Google Scholar]
  5. Antunes A., Rainey F. A., Nobre M. F., Schumann P., Ferreira A. M., Ramos A., Santos H., da Costa M. S. 2002; Leuconostoc ficulneum sp. nov., a novel lactic acid bacterium isolated from a ripe fig, and reclassification of Lactobacillus fructosus as Leuconostoc fructosum comb. nov.. Int J Syst Evol Microbiol 52:647–655[PubMed]
     [Google Scholar]
  6. Arun A. B., Chen W.-M., Lai W.-A., Chou J.-H., Shen F.-T., Rekha P. D., Young C.-C. 2009; Lutaonella thermophila gen. nov., sp. nov., a moderately thermophilic member of the family Flavobacteriaceae isolated from a coastal hot spring. Int J Syst Evol Microbiol 59:2069–2073 [CrossRef][PubMed]
     [Google Scholar]
  7. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef][PubMed]
     [Google Scholar]
  8. Bowman J. P., McCammon S. A., Brown J. L., Nichols P. D., McMeekin T. A. 1997; Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from antarctic lacustrine and sea ice habitats. Int J Syst Bacteriol 47:670–677 [CrossRef][PubMed]
     [Google Scholar]
  9. Bowman J. P., Nichols C. M., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Bacteriol 53:1343–1355 [CrossRef]
     [Google Scholar]
  10. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [CrossRef][PubMed]
     [Google Scholar]
  11. da Costa M. S., Nobre M. F., Wait R. 2006; Analysis of lipids from extremophilic bacteria. In Extremophiles, Methods in Microbiology vol. 35 pp. 127–159 Edited by Rainey F.A., Oren A. London: Academic Press;
     [Google Scholar]
  12. Degryse E., Glansdorff N., Piérard A. 1978; A comparative analysis of extreme thermophilic bacteria belonging to the genus Thermus . Arch Microbiol 117:189–196 [CrossRef][PubMed]
     [Google Scholar]
  13. Denger K., Warthmann R., Ludwig W., Schink B. 2002; Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium. Int J Syst Evol Microbiol 52:173–178[PubMed]
     [Google Scholar]
  14. França L., Rainey F. A., Nobre M. F., da Costa M. S. 2006; Tepidicella xavieri gen. nov., sp. nov., a betaproteobacterium isolated from a hot spring runoff. Int J Syst Evol Microbiol 56:907–912 [CrossRef][PubMed]
     [Google Scholar]
  15. Hudson J. A., Schofield K. M., Morgan H. W., Daniel R. M. 1989; Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. Int J Syst Bacteriol 39:485–487 [CrossRef]
     [Google Scholar]
  16. Lau K. W. K., Ng C. Y. M., Ren J., Lau S. C. L., Qian P.-Y., Wong P.-K., Lau T. C., Wu M. 2005; Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55:1051–1057 [CrossRef][PubMed]
     [Google Scholar]
  17. Lau K. W. K., Ren J., Wai N. L. M., Qian P.-Y., Wong P.-K., Wu M. 2006; Lishizhenia caseinilytica gen. nov., sp. nov., a marine bacterium of the phylum Bacteroidetes . Int J Syst Evol Microbiol 56:2317–2322 [CrossRef][PubMed]
     [Google Scholar]
  18. Lee D.-H., Choi E.-K., Moon S.-R., Ahn S., Lee Y. S., Jung J. S., Jeon C. O., Whang K.-S., Kahng H.-Y. 2010; Wandonia haliotis gen. nov., sp. nov., a marine bacterium of the family Cryomorphaceae, phylum Bacteroidetes . Int J Syst Evol Microbiol 60:510–514 [CrossRef][PubMed]
     [Google Scholar]
  19. Marteinsson V. T., Bjornsdottir S. H., Bienvenu N., Kristjannsson J. K., Birrien J.-L. 2010; Rhodothermus profundi sp. nov., a thermophilic bacterium isolated from a deep-sea hydrothermal vent in the Pacific Ocean. Int J Syst Evol Microbiol 60:2729–2734 [CrossRef]
     [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
     [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Rhee M. S., Frolova G. M., Falsen E., Mikhailov V. V., Bae K. S. 2004; Maribacter gen. nov., a new member of the family Flavobacteriaceae, isolated from marine habitats, containing the species Maribacter sedimenticola sp. nov., Maribacter aquivivus sp. nov., Maribacter orientalis sp. nov. and Maribacter ulvicola sp. nov.. Int J Syst Evol Microbiol 54:1017–1023 [CrossRef][PubMed]
     [Google Scholar]
  22. Nedashkovskaya O. I., Suzuki M., Lee J.-S., Lee K. C., Shevchenko L. S., Mikhailov V. V. 2009; Pseudozobellia thermophila gen. nov., sp. nov., a bacterium of the family Flavobacteriaceae, isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 59:806–810 [CrossRef][PubMed]
     [Google Scholar]
  23. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
     [Google Scholar]
  24. Nunes O. C., Manaia C. M., da Costa M. S., Santos H. 1995; Compatible solutes in the thermophilic bacteria Rhodothermus marinus and ‘Thermus thermophilus’ . Appl Environ Microbiol 61:2351–2357[PubMed]
     [Google Scholar]
  25. O’Sullivan L. A., Rinna J., Humphreys G., Weightman A. J., Fry J. C. 2005; Fluviicola taffensis gen. nov., sp. nov., a novel freshwater bacterium of the family Cryomorphaceae in the phylum ‘Bacteroidetes’. Int J Syst Evol Microbiol 55:2189–2194 [CrossRef][PubMed]
     [Google Scholar]
  26. Pires A. L., Albuquerque L., Tiago I., Nobre M. F., Empadinhas N., Veríssimo A., da Costa M. S. 2005; Meiothermus timidus sp. nov., a new slightly thermophilic yellow-pigmented species. FEMS Microbiol Lett 245:39–45 [CrossRef][PubMed]
     [Google Scholar]
  27. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:1088–1092 [CrossRef][PubMed]
     [Google Scholar]
  28. Silva Z., Horta C., da Costa M. S., Chung A. P., Rainey F. A. 2000; Polyphasic evidence for the reclassification of Rhodothermus obamensis Sako et al. 1996 as a member of the species Rhodothermus marinus Alfredsson et al. 1988. Int J Syst Evol Microbiol 50:1457–1461 [CrossRef][PubMed]
     [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp. 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  30. Sörbo B. 1987; Sulfate: turbidimetric and nephelometric methods. Methods Enzymol 143:3–6 [CrossRef][PubMed]
     [Google Scholar]
  31. Tenreiro S., Nobre M. F., Rainey F. A., Miguel C., da Costa M. S. 1997; Thermonema rossianum sp. nov., a new thermophilic and slightly halophilic species from saline hot springs in Naples, Italy. Int J Syst Bacteriol 47:122–126 [CrossRef][PubMed]
     [Google Scholar]
  32. Westley J. 1987; Thiocyanate and thiosulfate. Methods Enzymol 143:22–25 [CrossRef][PubMed]
     [Google Scholar]
  33. Williams R. A., da Costa M. S. 1992; The genus Thermus and related microorganisms. In The Prokaryotes, 2nd edn. pp. 3745–3753 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
     [Google Scholar]
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Schleiferia thermophila gen. nov., sp. nov., a slightly thermophilic bacterium of the phylum ‘Bacteroidetes’ and the proposal of Schleiferiaceae fam. nov.
Int J Syst Evol Microbiol 61, 2450 (2011); https://doi.org/10.1099/ijs.0.028852-0
/content/journal/ijsem/10.1099/ijs.0.028852-0
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