1887

Abstract

The taxonomic position of an actinomycete isolated from a hypermagnesian ultramafic soil was examined using a polyphasic approach. The strain, designated SBH OA6, was shown to have chemical and morphological properties typical of members of the genus . The organism was most closely associated with using 16S rRNA gene sequence data. It showed a distinctive set of phenotypic properties that distinguished it from representatives of all species with validly published names classified in the genus . The combined genotypic and phenotypic data show that strain SBH OA6 (=DSM 44717=NCIMB 13955) merits description as the type strain of a novel species, sp. nov.

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2004-03-01
2021-02-25
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References

  1. Albuquerque de Barros E. V. S., Manfio G. P., Ribeiro Maitan V., Mendes Bataus L. A., Kim S. B., Maldonado L. A., Goodfellow M. 2003; Nocardia cerradoensis sp. nov., a novel isolate from Cerrado soil in Brazil. Int J Syst Evol Microbiol 53:29–33 [CrossRef]
    [Google Scholar]
  2. Chun J. 1995; Computer-assisted classification and identification of actinomycetes . PhD thesis University of Newcastle; Newcastle upon Tyne, UK:
  3. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  4. Chun J., Blackall L. L., Kang S.-O., Hah Y. C., Goodfellow M. 1997; A proposal to reclassify Nocardia pinensis Blackall et al . as Skermania piniformis gen. nov., comb. nov.. Int J Syst Bacteriol 47:127–131 [CrossRef]
    [Google Scholar]
  5. Chun J., Seong C.-N., Bae K. S., Lee K.-J., Kang S.-O., Goodfellow M., Hah Y. C. 1998; Nocardia flavorosea sp. nov. Int J Syst Bacteriol 48:901–905 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1993 phylip (Phylogenetic Inference Package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  8. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284 [CrossRef]
    [Google Scholar]
  9. Goodfellow M., Isik K., Yates E. 1999; Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 312:NF8047–82
    [Google Scholar]
  10. Gordon R. E., Mihm J. M. 1962; Identification of Nocardia caviae (Erikson) nov. comb. Ann N Y Acad Sci 98:628–636
    [Google Scholar]
  11. Hamid M. E., Maldonado L., Sharaf Eldin G. S., Mohamed M. F., Saeed N. S., Goodfellow M. 2001; Nocardia africana sp. nov., a new pathogen isolated from patients with pulmonary infections. J Clin Microbiol 39:625–630 [CrossRef]
    [Google Scholar]
  12. Isik K., Chun J., Hah Y. C., Goodfellow M. 1999a; Nocardia salmonicida nom. rev., a fish pathogen. Int J Syst Bacteriol 49:833–837 [CrossRef]
    [Google Scholar]
  13. Isik K., Chun J., Hah Y. C., Goodfellow M. 1999b; Nocardia uniformis nom. rev. Int J Syst Bacteriol 49:1227–1230 [CrossRef]
    [Google Scholar]
  14. Jaffré T. 1976; Composition clinique et conditions de l'alimentation minérale des plantes sur roches ultrabasiques. Cah ORSTOM Ser Biol 11:53–63 (in French
    [Google Scholar]
  15. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  16. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  17. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Goodfellow M. 1999; Gordonia desulfuricans sp. nov., a benzothiophene-desulphurizing actinomycete. Int J Syst Bacteriol 49:1845–1851 [CrossRef]
    [Google Scholar]
  18. Kinoshita N., Homma Y., Igarashi M., Ikeno S., Hori M., Hamada M. 2001; Nocardia vinacea sp. nov. Actinomycetologica 15:1–5 [CrossRef]
    [Google Scholar]
  19. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  20. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  21. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  22. Maldonado L., Hookey J. V., Ward A. C., Goodfellow M. 2000; The Nocardia salmonicida clade, including descriptions of Nocardia cummidelens sp.nov., Nocardia fluminea sp. nov. and Nocardia soli sp. nov. Antonie van Leeuwenhoek 78:367–377 [CrossRef]
    [Google Scholar]
  23. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium , Nocardia , and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88:200–204 [CrossRef]
    [Google Scholar]
  24. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  25. Saintpierre D. 2001; Identification de souches originales d'actinomycétes isolées de sols ultramafiques de Nouvelle-Calédonie. Caractérisation chimique de quelques antibiotiques produits . PhD thesis Institut National Polytechnique de Toulouse; France: (in French
  26. Saintpierre D., Amir H., Pineau R., Sembiring L., Goodfellow M. 2003; Streptomyces yatensis sp. nov., a novel bioactive streptomycete isolated from a New-Caledonian ultramafic soil. Antonie van Leeuwenhoek 83:21–26 [CrossRef]
    [Google Scholar]
  27. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  28. Schaal K. P. 1985; Laboratory diagnosis of actinomycete diseases. In Chemical Methods in Bacterial Systematics pp  359–381 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  29. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  30. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  31. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  33. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  34. Yassin A. F., Rainey F. A., Mendrock U., Brzezinka H., Schaal K. P. 2000; Nocardia abscessus sp. nov. Int J Syst Evol Microbiol 50:1487–1493 [CrossRef]
    [Google Scholar]
  35. Yassin A. F., Rainey F. A., Steiner U. 2001; Nocardia ignorata sp. nov. Int J Syst Evol Microbiol 51:2127–2131 [CrossRef]
    [Google Scholar]
  36. Yassin A. F., Sträubler B., Schumann P., Schaal K. P. 2003; Nocardia puris sp. nov. Int J Syst Evol Microbiol 53:1595–1599 [CrossRef]
    [Google Scholar]
  37. Zhang J., Liu Z., Goodfellow M. 2003; Nocardia caishijiensis sp. nov., a novel soil actinomycete. Int J Syst Evol Microbiol 53:999–1004 [CrossRef]
    [Google Scholar]
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