1887

Abstract

A Gram-negative, rod-shaped and motile strain, designated CL-AS9, was isolated from polar seawater of the Arctic. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus , sharing 93.9 % and 93.7 % sequence similarities with the type strains of CL-CB221 and HAL40b, respectively. Phylogenetic analyses revealed that strain CL-AS9 formed a separate branch that was distinct from a clade comprising HAL40b, CL-CB221 and 5IX/A01/131. Cells of the strain grew optimally at 20–25 °C and pH 6.6–8.0 in the presence of 3–4 % (w/v) sea salts. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified aminophospholipid. The major quinone was ubiquinone 8. The major cellular fatty acids were Cω7 and/or iso-C 2-OH (23.1 %), Cω8 (22.1 %) and Cω7 (15.6 %). The genomic DNA G+C content was 53.6 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, we propose the name sp. nov. with the type strain CL-AS9 ( = KCCM 90094  = JCM 17304) and the reclassification of as a later heterotypic synonym of . We also provide emended descriptions of the genus and .

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2011-12-01
2019-10-17
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Two-dimensional thin-layer chromatograms of the polar lipids of (a) HAL40b , (b) 5IX/A01/131 and (c) strain CL-AS9 . PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; APL1 5, unidentified aminophospholipids.

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Cellular fatty acid compositions of strain CL-AS9 and other species in the clade [ PDF] (30 KB)

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