1887

Abstract

Two bacterial strains, EHS11 and EPSA11, which were isolated from the human oral cavity, were characterized in terms of phenotypic and biochemical characteristics, cellular fatty acid profiles and phylogenetic position based on 16S rRNA gene sequence analysis. 16S rRNA gene sequence analysis showed that each of the isolates belonged to a novel species of the genus . Strain EHS11 was related to (about 95 % similarity), whereas strain EPSA11 was related to (about 94 % similarity). Both strains were obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-negative rods. The cellular fatty acid composition of strain EPSA11 was very similar to that of JCM 8540. On the other hand, the cellular fatty acid composition of strain EHS11 was significantly different from those of other species. The predominant fatty acids in strain EHS11 are C 9, C and C 3-OH, whereas other species, except for JCM 8530, possess anteiso-C, iso-C 3-OH and C 9. The predominant fatty acids in JCM 8530 are anteiso-C, C and C 9. DNA–DNA hybridization experiments revealed a genomic distinction of strains EHS11 and EPSA11 from JCM 8530 and JCM 8540. On the basis of these data, two novel species are proposed: sp. nov. and sp. nov. The type strains of and are EHS11 (=JCM 12083=DSM 15611) and EPSA11 (=JCM 12084=DSM 15606), respectively.

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2004-05-01
2020-01-22
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Supplements

vol. , part 3, pp. 877 – 883

Phenotypic characteristics of two novel species and related species.

Menaquinone compositions of two novel species and related species.

DNA G+C contents and levels of DNA relatedness.

Phylogenetic tree showing the position of two novel species within the genus .

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