An actinomycete, strain RI109-Li102T, was isolated from a lichen sample obtained from Rishiri Island in Japan. Cells of strain RI109-Li102T were Gram-positive, aerobic and non-motile and formed bud-like spore chains. The isolate grew with 0–3 % (w/v) NaCl, at pH 5–9 and at 10–30 °C (optimum 30 °C). The whole-cell hydrolysate contained meso-diaminopimelic acid, arabinose and galactose. The predominant menaquinone was MK-8(H4) and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were iso-C16 : 0 and iso-C16 : 1 H. Comparative 16S rRNA gene sequence analysis revealed that strain RI109-Li102T was most closely related to Actinomycetospora corticicola 014-5T (99.0 % rRNA gene sequence similarity) and Actinomycetospora chiangmaiensis YIM 0006T (98.4 %). However, DNA–DNA hybridization assays, as well as physiological and biochemical analyses, showed that strain RI109-Li102T could be differentiated from its closest phylogenetic relatives. It is proposed that strain RI109-Li102T ( = NBRC 106356T = KCTC 19782T) be classified as the type strain of a novel species, with the name Actinomycetospora rishiriensis sp. nov.
GonzálezI.,
Ayuso-SacidoA.,
AndersonA.,
GenilloudO.2005; Actinomycetes isolated from lichens: evaluation of their diversity and detection of biosynthetic gene sequences. FEMS Microbiol Ecol 54:401–415 [View Article][PubMed]
GuindonS.,
LethiecF.,
DurouxP.,
GascuelO.2005; PhyML Online – a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Res 33:Web Server issueW557–W559 [View Article][PubMed]
HatanoK.,
NishiiT.,
KasaiH.2003; Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA–DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev.. Int J Syst Evol Microbiol 53:1519–1529 [View Article][PubMed]
HayakawaM.,
NonomuraH.1987; Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509 [View Article]
JiangY.,
WieseJ.,
TangS.-K.,
XuL.-H.,
ImhoffJ. F.,
JiangC.-L.2008; Actinomycetospora chiangmaiensis gen. nov., sp. nov., a new member of the family Pseudonocardiaceae
. Int J Syst Evol Microbiol 58:408–413 [View Article][PubMed]
KitouniM.,
BoudemaghA.,
OulmiL.,
ReghiouaS.,
BoughachicheF.,
ZerizerH.,
HamdikenH.,
CoubleA.,
MounieeD. et al.
other authors 2005; Isolation of actinomycetes producing bioactive substances from water, soil and tree bark samples of the north-east of Algeria. J Mycol Med 15:45–51[CrossRef]
LechevalierM. P.,
LechevalierH. A.1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Evol Microbiol 20:435–443
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
SasserM.1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
SchaalK. P.1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp. 359–381 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
TakahashiK.,
NeiM.2000; Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17:1251–1258[PubMed][CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
TamuraT.,
HatanoK.2001; Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov.. Int J Syst Evol Microbiol 51:2119–2125 [View Article][PubMed]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E. et al.
other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]