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Abstract

An actinomycete, strain RI109-Li102, was isolated from a lichen sample obtained from Rishiri Island in Japan. Cells of strain RI109-Li102 were Gram-positive, aerobic and non-motile and formed bud-like spore chains. The isolate grew with 0–3 % (w/v) NaCl, at pH 5–9 and at 10–30 °C (optimum 30 °C). The whole-cell hydrolysate contained -diaminopimelic acid, arabinose and galactose. The predominant menaquinone was MK-8(H) and the diagnostic phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol and diphosphatidylglycerol. The major cellular fatty acids were iso-C and iso-C H. Comparative 16S rRNA gene sequence analysis revealed that strain RI109-Li102 was most closely related to 014-5 (99.0 % rRNA gene sequence similarity) and YIM 0006 (98.4 %). However, DNA–DNA hybridization assays, as well as physiological and biochemical analyses, showed that strain RI109-Li102 could be differentiated from its closest phylogenetic relatives. It is proposed that strain RI109-Li102 ( = NBRC 106356  = KCTC 19782) be classified as the type strain of a novel species, with the name sp. nov.

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2011-11-01
2019-10-23
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