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Volume 62, Issue 2

Classification of avian haemolytic -like organisms (Bisgaard taxon 26) associated with anseriforme birds as sp. nov. Free

Abstract

Avian haemolytic -like organisms have tentatively been named Bisgaard taxon 26. Phenotypic information has been published on 65 strains of this taxon. In the current study, 31 isolates were selected for genotypic characterization. Thirty strains had the same sequence and only one strain diverged in 1 nt. The highest similarity to members of other taxa was 89.7 % to the type strain of subsp. and the similarity to the type strain of the type species, , was 88.2 %. The lowest 16S rRNA gene sequence similarity between strains of the group was determined in previous investigations to be 99.6 % and the highest similarities of 96.4 and 96.2 % outside the group were obtained to the reference strain of genomospecies 2 and to the type strain of subsp. , respectively; 95.8–95.3 % similarity was obtained with the type strain of . gene sequence similarities within the group were from 99.5 % (strains F66 and F64) to 99.8 % (strains F66 and F67) corresponding to genome similarities of 93.9–94.6 %, which are near the upper limit for species compared with other members of the . The highest similarity outside the group (83.4 %) was observed to the type strain of , whereas the similarity to the type strain of was 80.9 %, corresponding to genome similarities of 57.7 and 52.0 %, respectively. All isolates meet the phenotypic characters outlined for (urease-, phosphatase- and porphyrin-positive, indole-negative, acid production from fructose, sucrose, maltose and dextrin). β-Haemolysis of bovine blood is observed and isolates may demonstrate satellitic growth, referred to as V-factor or NAD requirement. Isolates have been obtained from the upper respiratory tract of web-footed birds in which they may cause sinusitis, conjunctivitis and septicaemia. Based on the characterization reported, it is proposed that the isolates belong to a novel species, sp. nov., which includes taxon 26 and a V-factor-dependent strain. The major fatty acids of the type strain are Cω7, C, C and C 3-OH and/or iso-C I, corresponding to the profile observed for the type strain of . Five to 12 characters separate from other taxa of , with being most closely related; can be differentiated from based on haemolysis, β-glucosidase, and production of acid from (−)--sorbitol, trehalose and glycosides. The type strain of is F66 ( = CCUG 60324 = CCM 7846), which was isolated from conjunctivitis in a White Pekin duck.

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2012-02-01
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Classification of avian haemolytic Actinobacillus-like organisms (Bisgaard taxon 26) associated with anseriforme birds as Actinobacillus anseriformium sp. nov.
Int J Syst Evol Microbiol 62, 352 (2012); https://doi.org/10.1099/ijs.0.028746-0
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