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Volume 62, Issue 1

sp. nov., a moderately thermophilic bacterium isolated from a hot spring Free

Abstract

A novel moderately thermophilic, red-pigmented bacterium, designated strain AZM34c11, was isolated from the bottom of a 1000 m-deep drilled well located in a Japanese hot spring. Cells were Gram-negative and grew optimally at 50 °C, at pH 7.6 and with 0–0.3 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence revealed that the isolate belonged to the genus . Levels of 16S rRNA gene sequence similarity between strain AZM34c11 and the type strains of recognized species were 88.2–94.8 %. Strain AZM34c11 was distinguished from recognized species by its cellular fatty acid profile: iso-C was one of the predominant components and hydroxy fatty acids were present only in trace amounts. The strain could also be differentiated based on its growth substrate preferences and characteristic enzyme reactions. On the basis of these results, strain AZM34c11 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AZM34c11 ( = NBRC 106114  = DSM 23238).

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2012-01-01
2024-04-18
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References

  1. Adachi J., Hasegawa M. 1995; Improved dating of the human/chimpanzee separation in the mitochondrial DNA tree: heterogeneity among amino acid sites. J Mol Evol 40:622–628 [View Article][PubMed]
     [Google Scholar]
  2. Albuquerque L., Ferreira C., Tomaz D., Tiago I., Veríssimo A., da Costa M. S., Nobre M. F. 2009; Meiothermus rufus sp. nov., a new slightly thermophilic red-pigmented species and emended description of the genus Meiothermus . Syst Appl Microbiol 32:306–313 [View Article][PubMed]
     [Google Scholar]
  3. Albuquerque L., Rainey F. A., Nobre M. F., da Costa M. S. 2010; Meiothermus granaticius sp. nov., a new slightly thermophilic red-pigmented species from the Azores. Syst Appl Microbiol 33:243–246 [View Article][PubMed]
     [Google Scholar]
  4. Castenholz R. W. 1969; Thermophilic blue-green algae and the thermal environment. Bacteriol Rev 33:476–504[PubMed]
     [Google Scholar]
  5. Chen M. Y., Lin G. H., Lin Y. T., Tsay S. S. 2002; Meiothermus taiwanensis sp. nov., a novel filamentous, thermophilic species isolated in Taiwan. Int J Syst Evol Microbiol 52:1647–1654 [View Article][PubMed]
     [Google Scholar]
  6. Chung A. P., Rainey F., Nobre M. F., Burghardt J., da Costa M. S. 1997; Meiothermus cerbereus sp. nov., a new slightly thermophilic species with high levels of 3-hydroxy fatty acids. Int J Syst Bacteriol 47:1225–1230 [View Article][PubMed]
     [Google Scholar]
  7. Dittmer J. C., Lester R. L. 1964; A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res 15:126–127[PubMed]
     [Google Scholar]
  8. Dobrogosz W. J. 1981; Enzymatic activity. In Manual of Methods for General Bacteriology pp. 365–392 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  9. Ekman J., Kosonen M., Jokela S., Kolari M., Korhonen P., Salkinoja-Salonen M. 2007; Detection and quantitation of colored deposit-forming Meiothermus spp. in paper industry processes and end products. J Ind Microbiol Biotechnol 34:203–211 [View Article][PubMed]
     [Google Scholar]
  10. Hamada M., Iino T., Iwami T., Harayama S., Tamura T., Suzuki K. 2010; Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.. J Gen Appl Microbiol 56:427–436 [View Article][PubMed]
     [Google Scholar]
  11. Hasegawa M., Kishino H., Yano T. A. 1985; Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174 [View Article][PubMed]
     [Google Scholar]
  12. Hattori S., Kamagata Y., Hanada S., Shoun H. 2000; Thermacetogenium phaeum gen. nov., sp. nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium. Int J Syst Evol Microbiol 50:1601–1609 [View Article][PubMed]
     [Google Scholar]
  13. Hudson J. A., Morgan H. W., Daniel R. M. 1986; A numerical classification of some Thermus isolates. J Gen Microbiol 132:531–540
     [Google Scholar]
  14. Kamagata Y., Mikami E. 1991; Isolation and characterization of a novel thermophilic Methanosaeta strain. Int J Syst Bacteriol 41:191–196 [View Article]
     [Google Scholar]
  15. Loginova L. G., Egorova L. A., Golovacheva R. S., Seregina L. M. 1984; Thermus ruber sp. nov., nom. rev.. Int J Syst Bacteriol 34:498–499 [View Article]
     [Google Scholar]
  16. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
     [Google Scholar]
  17. Manaia C. M., da Costa M. S. 1991; Characterization of halotolerant Thermus isolates from shallow marine hot springs on S. Miguel, Azores. J Gen Microbiol 137:2643–2648 [CrossRef]
     [Google Scholar]
  18. Mori K., Maruyama A., Urabe T., Suzuki K., Hanada S. 2008a; Archaeoglobus infectus sp. nov., a novel thermophilic, chemolithoheterotrophic archaeon isolated from a deep-sea rock collected at Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean. Int J Syst Evol Microbiol 58:810–816 [View Article][PubMed]
     [Google Scholar]
  19. Mori K., Sunamura M., Yanagawa K., Ishibashi J., Miyoshi Y., Iino T., Suzuki K., Urabe T. 2008b; First cultivation and ecological investigation of a bacterium affiliated with the candidate phylum OP5 from hot springs. Appl Environ Microbiol 74:6223–6229 [View Article][PubMed]
     [Google Scholar]
  20. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161[PubMed] [CrossRef]
     [Google Scholar]
  21. Nobre M. F., da Costa M. S. 2001; Genus II. Meiothermus Nobre, Trüper and da Costa, 1996b, 605VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 1 pp. 414–420 Edited by Boone D. R., Castenholtz R. W., Garrity G. M. New York: Springer;
     [Google Scholar]
  22. Nobre M. F., Trüper H. G., da Costa M. S. 1996; Transfer of Thermus ruber (Loginova et al. 1984), Thermus silvanus (Tenreiro et al. 1995), and Thermus chliarophilus (Tenreiro et al. 1995) to Meiothermus gen. nov. as Meiothermus ruber comb. nov., Meiothermus silvanus comb. nov., and Meiothermus chliarophilus comb. nov., respectively, and emendation of the genus Thermus . Int J Syst Bacteriol 46:604–606 [View Article]
     [Google Scholar]
  23. Pires A. L., Albuquerque L., Tiago I., Nobre M. F., Empadinhas N., Veríssimo A., da Costa M. S. 2005; Meiothermus timidus sp. nov., a new slightly thermophilic yellow-pigmented species. FEMS Microbiol Lett 245:39–45 [View Article][PubMed]
     [Google Scholar]
  24. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  25. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  26. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp. 409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  27. Tamaki H., Hanada S., Kamagata Y., Nakamura K., Nomura N., Nakano K., Matsumura M. 2003; Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53:519–526 [View Article][PubMed]
     [Google Scholar]
  28. Tenreiro S., Nobre M. F., da Costa M. S. 1995; Thermus silvanus sp. nov. and Thermus chliarophilus sp. nov., two new species related to Thermus ruber but with lower growth temperatures. Int J Syst Bacteriol 45:633–639 [View Article][PubMed]
     [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  30. Zhang X. Q., Zhang W. J., Wei B. P., Xu X. W., Zhu X. F., Wu M. 2010; Meiothermus cateniformans sp. nov., a slightly thermophilic species from north-eastern China. Int J Syst Evol Microbiol 60:840–844 [View Article][PubMed]
     [Google Scholar]
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Meiothermus hypogaeus sp. nov., a moderately thermophilic bacterium isolated from a hot spring
Int J Syst Evol Microbiol 62, 112 (2012); https://doi.org/10.1099/ijs.0.028654-0
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