1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 62, Issue Pt_8

sp. nov., isolated from a solar saltern Free

Abstract

A taxonomic study was performed on two isolates, designated strains MK-B5 and MK-B7, isolated from sediment of a solar saltern pond in Gomso Bay, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that strains MK-B5 and MK-B7 belong to the and are related most closely to JCM 11575 ( = E1L3A) (96.3 and 96.5 % similarity, respectively), KCCM 90064 ( = CL-ES53) (95.6 and 95.6 %) and JCM 115514 ( = EPR70) (95.1 and 95.3 %). The level of 16S rRNA gene sequence similarity between strains MK-B5 and MK-B7 was 99.8 %. The G+C contents of their genomic DNAs were 63.4 and 63.6 mol%, respectively, and the major respiratory quinone was ubiquinone-8. DNA–DNA relatedness between strains MK-B5 and MK-B7 was 98 %, indicating that the two isolates represent a single species. However, the level of DNA–DNA relatedness between the two isolates and E1L3A (26.4–30.8 %) indicates that they represent a novel species. Strains MK-B5 and MK-B7 possessed C, C and Cω8 cyclo as major fatty acids. The two isolates were Gram-stain-negative, strictly aerobic, short rod-shaped and motile. They grew at 10–40 °C (optimum, 35–37 °C), at pH 5.0–8.5 (optimum, 7.0–7.5) and with 5–25 % (w/v) NaCl (optimum, 15 % NaCl). On the basis of phenotypic and phylogenetic analyses, strains MK-B5 and MK-B7 are thus considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MK-B5 ( = KCTC 23198 = JCM 17073).

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Funding
This study was supported by the:
  • National Research Foundation of Korea (NRF) (Award 2009-0087901)
© 2012 IUMS
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2012-08-01
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References

  1. Antunes A., Eder W., Fareleira P., Santos H., Huber R. 2003; Salinisphaera shabanensis gen. nov., sp. nov., a novel, moderately halophilic bacterium from the brine–seawater interface of the Shaban Deep, Red Sea. Extremophiles 7:29–34[PubMed]
     [Google Scholar]
  2. Atlas R. M. 2004 Handbook of Microbiological Media, 3rd edn. Boca Raton, FL: CRC Press; [View Article]
     [Google Scholar]
  3. Bae G. D., Hwang C. Y., Kim H. M., Cho B. C. 2010; Salinisphaera dokdonensis sp. nov., isolated from surface seawater. Int J Syst Evol Microbiol 60:680–685 [View Article][PubMed]
     [Google Scholar]
  4. Chun J., Lee J. H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  5. Crespo-Medina M., Chatziefthimiou A., Cruz-Matos R., Pérez-Rodríguez I., Barkay T., Lutz R. A., Starovoytov V., Vetriani C. 2009; Salinisphaera hydrothermalis sp. nov., a mesophilic, halotolerant, facultatively autotrophic, thiosulfate-oxidizing gammaproteobacterium from deep-sea hydrothermal vents, and emended description of the genus Salinisphaera . Int J Syst Evol Microbiol 59:1497–1503 [View Article][PubMed]
     [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
     [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
     [Google Scholar]
  8. Forbes L. 1981; Rapid flagella stain. J Clin Microbiol 13:807–809[PubMed]
     [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773 [View Article][PubMed]
     [Google Scholar]
  10. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
     [Google Scholar]
  11. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
     [Google Scholar]
  12. Ito T., Sugita K., Yumoto I., Nodasaka Y., Okabe S. 2005; Thiovirga sulfuroxydans gen. nov., sp. nov., a chemolithoautotrophic sulfur-oxidizing bacterium isolated from a microaerobic waste-water biofilm. Int J Syst Evol Microbiol 55:1059–1064 [View Article][PubMed]
     [Google Scholar]
  13. Kelly D. P., Wood A. P. 2000; Reclassification of some species of Thiobacillus to the newly designated genera Acidithiobacillus gen. nov., Halothiobacillus gen. nov. and Thermithiobacillus gen. nov.. Int J Syst Evol Microbiol 50:511–516 [View Article][PubMed]
     [Google Scholar]
  14. Kidd K. K., Sgaramella-Zonta L. A. 1971; Phylogenetic analysis: concepts and methods. Am J Hum Genet 23:235–252[PubMed]
     [Google Scholar]
  15. Kimura M. 1983 The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; [CrossRef]
     [Google Scholar]
  16. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
     [Google Scholar]
  17. MIDI 1999 Sherlock Microbial Identification System, Operating Manual version 3.0 Newark, DE: MIDI;
     [Google Scholar]
  18. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp. 21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  19. Park S. J., Kang C. H., Rhee S. K. 2006; Characterization of the microbial diversity in a Korean solar saltern by 16S rRNA gene analysis. J Microbiol Biotechnol 16:1640–1645
     [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  21. Sievert S. M., Heidorn T., Kuever J. 2000; Halothiobacillus kellyi sp. nov., a mesophilic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium isolated from a shallow-water hydrothermal vent in the Aegean Sea, and emended description of the genus Halothiobacillus . Int J Syst Evol Microbiol 50:1229–1237 [View Article][PubMed]
     [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  25. Tittsler R. P., Sandholzer L. A. 1936; The use of semi-solid agar for the detection of bacterial motility. J Bacteriol 31:575–580[PubMed]
     [Google Scholar]
  26. Vreeland R. H., Litchfield C. D., Martin E. L., Elliot E. 1980; Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30:485–495 [View Article]
     [Google Scholar]
  27. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  28. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703[PubMed]
     [Google Scholar]
  29. Widdel F., Bak F. 1992; Gram-negative mesophilic sulfate-reducing bacteria. In The Prokaryotes, 2nd edn. pp. 3352–3378 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. Berlin: Springer; [CrossRef]
     [Google Scholar]
  30. Wolin E. A., Wolin M. J., Wolfe R. S. 1963; Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886[PubMed]
     [Google Scholar]
  31. Yi H., Chang Y. H., Oh H. W., Bae K. S., Chun J. 2003; Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. Int J Syst Evol Microbiol 53:1013–1018 [View Article][PubMed]
     [Google Scholar]
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Salinisphaera orenii sp. nov., isolated from a solar saltern
Int J Syst Evol Microbiol 62, 1877 (2012); https://doi.org/10.1099/ijs.0.028647-0
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