1887

Abstract

TXW-1, a strain isolated in March 1998 from an adult male tick feeding on a coyote from Webb county, Texas, USA, was characterized by using randomly amplified polymorphic DNA (RAPD) analysis, RFLP and sequence analysis of and (16S rRNA gene), DNA–DNA hybridization analysis, SDS-PAGE and Western blotting with mAbs. It shows different banding patterns in RFLP analysis of and forms distinct branches in phylogenetic analysis derived from and genes. It differs from other borreliae based on the banding patterns obtained by RAPD analysis. This strain contains a small, 38-kDa endoflagellar protein. DNA–DNA hybridization experiments revealed that the levels of DNA reassociation between TXW-1 and previously described relapsing fever borreliae were 38·64 % (), 38·40 % (), 7·39 % () and 18·30 % (). However, the level of DNA relatedness between and was 78·78 %. Sequence analyses of and genes indicate that the similarities of nucleotide sequences among TXW-1 and or are less than that between and , and that the genetic distances among TXW-1 and or are greater than that between and . TXW-1 lacks an gene. Electron microscope observations showed that this spirochaete had different morphological structures compared to previously described relapsing fever borreliae. All the results obtained from the above-mentioned analyses indicate that TXW-1 is different from other described species and that it represents a novel species of . We have been unable to revive frozen cultures and so can not meet the requirements of the Bacteriological Code to deposit viable type material at two different culture collections. Therefore we use the designation; based on these results, the species ‘ Borrelia texasensis' is proposed.

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2005-03-01
2019-12-07
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vol. , part 2, pp. 685 - 693

Scanning electron micrograph of strain TXW-1.



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