1887

Abstract

Two isolates of mulberry-pathogenic bacteria isolated from diseased mulberry roots were investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with gene sequence analysis allocated strains R18-2 and R3-3 to the genus , with , , , subsp. , subsp. and as their closest relatives. Cells of the isolates were Gram-negative, facultatively anaerobic rods, 0.3–1.0 µm wide and 0.8–2.0 µm long, with peritrichous flagella, showing a DNA G+C content of 55.1±0.5 mol%. Calculation of a similarity index based on phenotypic features and fatty acid methyl ester (FAME) analysis suggested that these isolates are members of or or a closely related species. Biochemical data revealed that the isolates could be differentiated from their nearest neighbours by the presence of lysine decarboxylase activity and their ability to utilize -arabitol. DNA–DNA relatedness also distinguished the two isolates from phylogenetically closely related strains. Based on these data, it is proposed that the isolates represent a novel species of the genus , named sp. nov. The type strain is R18-2 ( = CGMCC 1.10322  = LMG 25706).

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2011-11-01
2019-10-20
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vol. , part 11, pp. 2769 - 2774

Phylogenetic relationships based on sequences determined by the neighbour-joining method with the program package MEGA 4.0.

Cellular fatty acid compositions of strains R18-2 and R3-3 and type strains of related species of the genus .

DNA–DNA hybridization between strain R18-2 and its closest relatives.

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