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Abstract

Taxonomic studies were performed on 36 strains that were isolated from microbial mats in Antarctic lakes of the Vestfold Hills, the Larsemann Hills and the McMurdo Dry Valleys. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains are related to members of the genus ; sequence similarity values with their nearest phylogenetic neighbours ranged from 96·8 to 98·5 %. Results of DNA–DNA hybridization and comparison of repetitive extragenic palindromic DNA-PCR fingerprinting patterns revealed that these strains are members of three distinct species. Genotypic results, together with phenotypic characteristics, allowed the differentiation of these species from related species with validly published names. The isolates are Gram-negative, chemoheterotrophic, rod-shaped cells that are psychrophilic and moderately halotolerant; their DNA G+C contents range from 33·1 to 34·5 mol%. Their whole-cell fatty acid profiles are similar and include C, anteiso-C, iso-C, C 6, iso-C, iso-C 3-OH and summed feature 3 (which comprises iso-C 2-OH, C 7 or both) as major fatty acid components. On the basis of these results, three novel species are proposed, namely sp. nov. (consisting of 14 strains, with LMG 21915=DSM 15718 as the type strain), sp. nov. (consisting of three strains, with LMG 21919=CIP 108161 as the type strain) and sp. nov. (consisting of 19 strains, with LMG 21922=DSM 15719 as the type strain).

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2004-01-01
2020-01-25
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vol. , part 1, pp. 85 – 92

Neighbour-joining dendrogram showing the estimated phylogenetic relationships of the novel species.

Differential phenotypic characteristics of species.

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