1887

Abstract

A moderately halophilic and alkalitolerant bacterium, designated strain HN30, was isolated from garden soil in Japan. Cells of strain HN30 were motile, endospore-forming, aerobic, rod-shaped and Gram-positive, and contained A1γ -diaminopimelic acid-type murein. Growth occurred in 7–23 % (w/v) NaCl (optimum, 10–15 %, w/v), at pH 6.5–10.0 (optimum, pH 8.0–8.5) and at 20–40 °C (optimum, 30 °C). The isoprenoid quinone was menaquinone-7. The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. The major cellular fatty acids were anteiso-C, anteiso-C, iso-C and C. The DNA G+C content of strain HN30 was 47 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HN30 was most closely related to BH1 (93 % sequence similarity). 16S rRNA gene sequence similarities with other recognized species were less than 89 %. Phylogenetic and phenotypic characteristics indicated that strain HN30 represents a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed; the type strain is HN30 ( = JCM 15649 = DSM 21771).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.028506-0
2012-02-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/2/289.html?itemId=/content/journal/ijsem/10.1099/ijs.0.028506-0&mimeType=html&fmt=ahah

References

  1. Albert R. A., Archambault J., Lempa M., Hurst B., Richardson C., Gruenloh S., Duran M., Worliczek H. L., Huber B. E.. & other authors ( 2007;). Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov.. Int J Syst Evol Microbiol 57:, 2729–2737. [CrossRef][PubMed]
    [Google Scholar]
  2. Amoozegar M. A., Sánchez-Porro C., Rohban R., Hajighasemi M., Ventosa A.. ( 2009;). Piscibacillus halophilus sp. nov., a moderately halophilic bacterium from a hypersaline Iranian lake. . Int J Syst Evol Microbiol 59:, 3095–3099. [CrossRef][PubMed]
    [Google Scholar]
  3. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D.. ( 1991;). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13:, 202–206. [CrossRef]
    [Google Scholar]
  4. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A.. ( 2007;). Salsuginibacillus kocurii gen. nov., sp. nov., a moderately halophilic bacterium from soda-lake sediment. . Int J Syst Evol Microbiol 57:, 2381–2386. [CrossRef][PubMed]
    [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W.. ( 2007;). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef][PubMed]
    [Google Scholar]
  6. Echigo A., Hino M., Fukushima T., Mizuki T., Kamekura M., Usami R.. ( 2005;). Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm). . Saline Syst 1:, 8. [CrossRef][PubMed]
    [Google Scholar]
  7. Echigo A., Fukushima T., Mizuki T., Kamekura M., Usami R.. ( 2007;). Halalkalibacillus halophilus gen. nov., sp. nov., a novel moderately halophilic and alkaliphilic bacterium isolated from a non-saline soil sample in Japan. . Int J Syst Evol Microbiol 57:, 1081–1085. [CrossRef][PubMed]
    [Google Scholar]
  8. Echigo A., Minegishi H., Mizuki T., Kamekura M., Usami R.. ( 2010;). Geomicrobium halophilum gen. nov., sp. nov., a moderately halophilic and alkaliphilic bacterium isolated from soil. . Int J Syst Evol Microbiol 60:, 990–995. [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Felsenstein J.. ( 2002;). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA. .
  11. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H. J., Tindall B. J.. ( 2006;). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56:, 781–786. [CrossRef][PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  13. L’Haridon S., Miroshnichenko M. L., Kostrikina N. A., Tindall B. J., Spring S., Schumann P., Stackebrandt E., Bonch-Osmolovskaya E. A., Jeanthon C.. ( 2006;). Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, nitrate-reducing bacterium from deep-sea hydrothermal vents. . Int J Syst Evol Microbiol 56:, 1047–1053. [CrossRef][PubMed]
    [Google Scholar]
  14. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A.. & other authors ( 2007;). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef][PubMed]
    [Google Scholar]
  15. Lim J. M., Jeon C. O., Lee S. M., Lee J. C., Xu L. H., Jiang C. L., Kim C. J.. ( 2006;). Bacillus salarius sp. nov., a halophilic, spore-forming bacterium isolated from a salt lake in China. . Int J Syst Evol Microbiol 56:, 373–377. [CrossRef][PubMed]
    [Google Scholar]
  16. Lipman D. J., Pearson W. R.. ( 1985;). Rapid and sensitive protein similarity searches. . Science 227:, 1435–1441. [CrossRef][PubMed]
    [Google Scholar]
  17. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L.. & other authors ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef][PubMed]
    [Google Scholar]
  18. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenegger M., Neumaier J., Bachleitner M., Schleifer K. H.. ( 1998;). Bacterial phylogeny based on comparative sequence analysis. . Electrophoresis 19:, 554–568. [CrossRef][PubMed]
    [Google Scholar]
  19. Márquez M. C., Carrasco I. J., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A.. ( 2008;). Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. . Int J Syst Evol Microbiol 58:, 1922–1926. [CrossRef][PubMed]
    [Google Scholar]
  20. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  21. Miyazaki S., Sugawara H., Gojobori T., Tateno Y.. ( 2003;). DNA Data Bank of Japan (DDBJ) in XML. . Nucleic Acids Res 31:, 13–16. [CrossRef][PubMed]
    [Google Scholar]
  22. Nielsen P., Rainey F. A., Outtrup H., Priest F. G., Fritze D.. ( 1994;). Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus. . FEMS Microbiol Lett 117:, 61–65. [CrossRef]
    [Google Scholar]
  23. Oren A., Ventosa A., Grant W. D.. ( 1997;). Proposed minimal standards for description of new taxa in the order Halobacteriales. . Int J Syst Bacteriol 47:, 233–238. [CrossRef]
    [Google Scholar]
  24. Pearson W. R., Lipman D. J.. ( 1988;). Improved tools for biological sequence comparison. . Proc Natl Acad Sci U S A 85:, 2444–2448. [CrossRef][PubMed]
    [Google Scholar]
  25. Ren P. G., Zhou P. J.. ( 2005;). Tenuibacillus multivorans gen. nov., sp. nov., a moderately halophilic bacterium isolated from saline soil in Xin-Jiang, China. . Int J Syst Evol Microbiol 55:, 95–99. [CrossRef][PubMed]
    [Google Scholar]
  26. Saito H., Miura K. I.. ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. . Biochim Biophys Acta 72:, 619–629. [CrossRef][PubMed]
    [Google Scholar]
  27. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Schleifer K. H.. ( 1985;). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  29. Schleifer K. H., Kandler O.. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36:, 407–477.[PubMed]
    [Google Scholar]
  30. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Völker H., Thomm M.. ( 2001;). Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-d-Glu-type peptidoglycan. . Int J Syst Evol Microbiol 51:, 425–431.[PubMed]
    [Google Scholar]
  31. Smibert R. M., Krieg N. R.. ( 1981;). General characterization. . In Manual of Methods for General Microbiology, pp. 409–443. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  32. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  33. Stamatakis A., Ludwig T., Meier H.. ( 2005;). RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. . Bioinformatics 21:, 456–463. [CrossRef][PubMed]
    [Google Scholar]
  34. Tamaoka J.. ( 1986;). Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography. . Methods Enzymol 123:, 251–256. [CrossRef][PubMed]
    [Google Scholar]
  35. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  36. Usami R., Echigo A., Fukushima T., Mizuki T., Yoshida Y., Kamekura M.. ( 2007;). Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan. . Int J Syst Evol Microbiol 57:, 770–774. [CrossRef][PubMed]
    [Google Scholar]
  37. Ventosa A., Nieto J. J., Oren A.. ( 1998;). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62:, 504–544.[PubMed]
    [Google Scholar]
  38. Wainø M., Tindall B. J., Schumann P., Ingvorsen K.. ( 1999;). Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. Int J Syst Bacteriol 49:, 821–831. [CrossRef][PubMed]
    [Google Scholar]
  39. Wang Q. F., Li W., Liu Y. L., Cao H. H., Li Z., Guo G. Q.. ( 2007;). Bacillus qingdaonensis sp. nov., a moderately haloalkaliphilic bacterium isolated from a crude sea-salt sample collected near Qingdao in eastern China. . Int J Syst Evol Microbiol 57:, 1143–1147. [CrossRef][PubMed]
    [Google Scholar]
  40. Xue Y., Ventosa A., Wang X., Ren P., Zhou P., Ma Y.. ( 2008;). Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China. . Int J Syst Evol Microbiol 58:, 2828–2832. [CrossRef][PubMed]
    [Google Scholar]
  41. Yoon J. H., Kang K. H., Park Y. H.. ( 2002;). Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. . Int J Syst Evol Microbiol 52:, 2043–2048. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.028506-0
Loading
/content/journal/ijsem/10.1099/ijs.0.028506-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error