1887

Abstract

An actinomycete strain, isolated from a Mata Atlântica soil sample, showing cellulolytic activity was subjected to polyphasic taxonomic characterization to determine its identity. Strain M7a presented morphological and chemotaxonomic characteristics consistent with its assignment to the genus . Phylogenetic analysis of its 16S rDNA sequence revealed that the strain differed from described streptomycetes available in the public databases; the most closely related species was , with 98·4 % nucleotide similarity. It also differed from other cellulolytic strains in its phenotypic characteristics. It is therefore proposed that strain M7a, a cellulolytic strain with biotechnological potential, represents a novel species, named sp. nov. The type strain is M7a (=CBMAI 0498=CIP 107837=NRRL B-24297).

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2004-07-01
2024-12-06
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Bull A. T., Goodfellow M., Slater J. H. 1992; Biodiversity as a source of innovation in biotechnology. Annu Rev Microbiol 46:219–252 [CrossRef]
    [Google Scholar]
  3. Hopkins D. W., MacNaughton S. J., O'Donnell A. G. 1991; A dispersion and differential centrifugation technique for representatively sampling microorganisms from soil. Soil Biol Biochem 23:217–225 [CrossRef]
    [Google Scholar]
  4. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  5. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 mega2: Molecular Evolutionary Genetics Analysis software Tempe, AZ: Arizona State University;
    [Google Scholar]
  6. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: John Wiley;
    [Google Scholar]
  7. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [CrossRef]
    [Google Scholar]
  8. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  9. Lepage G., Roy C. C. 1984; Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res 25:1391–1396
    [Google Scholar]
  10. Li X. 1997; Streptomyces cellulolyticus sp. nov., a new cellulolytic member of the genus Streptomyces . Int J Syst Bacteriol 47:443–445 [CrossRef]
    [Google Scholar]
  11. Locci R. 1989; Streptomyces and related genera. In Bergey's Manual of Systematic Bacteriology vol 4 pp  2451–2506 Edited by Williams S. T., Sharp M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project. Nucleic Acids Res 25:109–111 [CrossRef]
    [Google Scholar]
  13. Manfio G. P., Atalan E., Zakrzewska-Czerwinska J., Mordarski M., Rodríguez C., Collins M. D., Goodfellow M. 2003; Classification of novel soil streptomycetes as Streptomyces aureus sp.nov., Streptomyces laceyi sp. nov. and Streptomyces sanglieri sp. nov. Antonie van Leeuwenhoek 83:245–255 [CrossRef]
    [Google Scholar]
  14. Okami Y., Hotta K. 1988; Search and discovery of new antibiotics. In Actinomycetes in Biotechnology pp  37–67 Edited by Goodfellow M., Williams S. T., Mordarski M. London: Academic Press;
    [Google Scholar]
  15. Paim S., Linhares L. F., Mangrich A. S., Martin J. P. 1990; Characterization of fungal melanins and soil humic acids by chemical analysis and infrared spectroscopy. Biol Fertil Soils 10:72–76
    [Google Scholar]
  16. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  18. Semêdo L. T. A. S., Gomes R. C., Bon E. P. S., Soares R. M. S., Linhares L. F., Coelho R. R. R. 2000; Endocellulase and exocellulase activities of two Streptomyces strains isolated from a forest soil. Appl Biochem Biotechnol 84:86267–276
    [Google Scholar]
  19. Semêdo L. T. A. S., Linhares A. A., Gomes R. C., Manfio G. P., Alviano C. S., Linhares L. F., Coelho R. R. R. 2001; Isolation and characterization of actinomycetes from Brazilian tropical soils. Microbiol Res 155:291–299 [CrossRef]
    [Google Scholar]
  20. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  21. Stackebrandt E., Liesack W., Witt D. 1992; Ribosomal RNA and rDNA sequence analyses. Gene 115:255–260 [CrossRef]
    [Google Scholar]
  22. Tamura K., Nei M. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal_w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  24. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983a; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  25. Williams S. T., Goodfellow M., Wellington E. M. H., Vickers J. C., Alderson G., Sneath P. H. A., Sackin M. J., Mortimer A. M. 1983b; A probability matrix for identification of some streptomycetes. J Gen Microbiol 129:1815–1830
    [Google Scholar]
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