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Abstract

A Gram-reaction-positive, non-motile, non-spore-forming, aerobic rod, designated BXN5-15, was isolated from the soil of a ginseng field on Baekdu Mountain in China. Strain BXN5-15 grew optimally at 30 °C and pH 6.0–7.0 in the absence of NaCl on R2A agar. Strain BXN5-15 displayed β-glucosidase activity, which allowed it to transform ginsenoside Rb (one of the dominant active components of ginseng) via Rd to minor ginsenoside F. On the basis of 16S rRNA gene sequence analysis, strain BXN5-15 was shown to belong to the genus . The closest phylogenetic relatives were Gsoil 355 (98.4 % 16S rRNA gene sequence similarity) and B33D1 (96.4 %). Lower sequence similarities (<96.0 %) were found with all of the other recognized members of the order . The predominant quinone was MK-7(H). The major fatty acids (>10 %) were Cω9, iso-C and Cω6,9,12. The G+C content of the genomic DNA was 70.6 mol%. DNA−DNA relatedness between strain BXN5-15 and KCTC 12628 was 23.3 %. On the basis of genotypic, phenotypic and chemotaxonomic data, strain BXN5-15 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is BXN5-15 ( = KACC 20671  = LMG 24459).

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2011-11-01
2020-01-28
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Transmission electron micrograph of negatively stained cells of sp. nov. BXN5-15 . Bar, 0.2 μm.

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Cellular fatty acid composition (%) of strain BXN5-15 and type strains of species of the genus . [PDF](50KB)

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