1887

Abstract

Ninety-five rhizobial strains isolated from growing in the northern regions of China were classified into three main groups, candidate species I, II and III, based on a polyphasic approach. Comparative analysis of full-length 16S rRNA gene sequences of representative strains showed that candidate species I and II were , while candidate species III, which consisted of non-nodulating strains, was closely related to . The phylogenetic relationships of the three candidate species and some related strains were also confirmed by the sequencing of genes, which were used as an alternative chromosomal marker. The DNA–DNA relatedness was between 11·3 and 47·1 % among representative strains of candidate species I and II and the type strains of defined species. Candidate III had DNA relatedness of between 4·3 and 25·2 % with type strains of and . Two novel species are proposed to accommodate candidate species I and II, sp. nov. (type strain, SDW014=CCBAU 11014=HAMBI 2582) and sp. nov. (type strain, SDW018=CCBAU 11018=HAMBI 2583), respectively. At least two distinct sequences were identified among the strains. The numerically dominant sequence type was most similar to that from the type strain and was identified in strains belonging to the two novel species as well as other, as yet, undefined genome types. Host range studies indicate that the different sequences correlate with different host ranges. Further comparative studies with the defined species are needed to clarify the taxonomic identity of candidate species III.

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2004-11-01
2019-12-05
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References

  1. Allen, O. N. & Allen, E. K. ( 1981; ). The Leguminoseae: a Source Book of Characteristics, Uses, and Nodulation. Madison, WI: University of Wisconsin Press.
  2. Chen, W. X., Yan, G. H. & Li, J. L. ( 1988; ). Numerical taxonomic study of fast-growing soybean rhizobia and a proposal that Rhizobium fredii be assigned to Sinorhizobium gen. nov. Int J Syst Bacteriol 38, 392–397.[CrossRef]
    [Google Scholar]
  3. Chen, W. X., Li, G. S., Qi, Y. L., Wang, E. T., Yuan, H. L. & Li, J. L. ( 1991; ). Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int J Syst Bacteriol 41, 275–280.[CrossRef]
    [Google Scholar]
  4. Chen, W. X., Wang, E. T., Wang, S. Y., Li, Y. B., Chen, X. Q. & Li, Y. ( 1995; ). Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People's Republic of China. Int J Syst Bacteriol 45, 153–159.[CrossRef]
    [Google Scholar]
  5. Chen, W.-X., Tan, Z.-Y., Gao, J.-L., Li, Y. & Wang, E.-T. ( 1997; ). Rhizobium hainanense sp. nov., isolated from tropical legumes. Int J Syst Bacteriol 47, 870–873.[CrossRef]
    [Google Scholar]
  6. de Lajudie, P., Willems, A., Nick, G. & 9 other authors ( 1998; ). Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. Int J Syst Bacteriol 48, 369–382.[CrossRef]
    [Google Scholar]
  7. de Lajudie, P., Willems, A., Nick, G. & 8 other authors ( 1999; ). Agrobacterium bv. 1 strains isolated from tropical legumes. Syst Appl Microbiol 22, 119–132.[CrossRef]
    [Google Scholar]
  8. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). Quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  9. Gao, J. L., Sun, J. G., Li, Y., Wang, E. T. & Chen, W. X. ( 1994; ). Numerical taxonomy and DNA relatedness of tropical rhizobia isolated from Hainan Province, China. Int J Syst Bacteriol 44, 151–158.[CrossRef]
    [Google Scholar]
  10. Gao, J. L., Terefework, Z. D., Chen, W. X. & Lindström, K. ( 2001; ). Genetic diversity of rhizobia isolated from Astragalus adsurgens growing in different geographical regions of China. J Biotechnol 91, 155–168.[CrossRef]
    [Google Scholar]
  11. Gaunt, M. W., Turner, S. L., Rigottier-Gois, L., Lloyd-Macgilp, S. A. & Young, J. P. W. ( 2001; ). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51, 2037–2048.[CrossRef]
    [Google Scholar]
  12. Graham, P. H., Sadowsky, M. J., Keyser, H. H. & 8 other authors ( 1991; ). Proposed minimal standards for the description of new genera and species of root- and stem-nodulating bacteria. Int J Syst Bacteriol 41, 582–587.[CrossRef]
    [Google Scholar]
  13. Guo, X. W., Zhang, X. X., Zhang, Z. M. & Li, F. D. ( 1999; ). Characterization of Astragalus sinicus rhizobia by restriction fragment length polymorphism analysis of chromosomal and nodulation genes regions. Curr Microbiol 39, 358–364.[CrossRef]
    [Google Scholar]
  14. Hurek, T., Wagner, B. & Reinhold-Hurek, B. ( 1997; ). Identification of N2-fixing plant- and fungus-associated Azoarcus species by PCR-based genomic fingerprints. Appl Environ Microbiol 63, 4331–4339.
    [Google Scholar]
  15. Jarvis, B. D. W., van Berkum, P., Chen, W. X., Nour, S. M., Fernandez, M. P., Cleyet-Marel, J. C. & Gillis, M. ( 1997; ). Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47, 895–898.[CrossRef]
    [Google Scholar]
  16. Laguerre, G., van Berkum, P., Amarger, N. & Prevost, D. ( 1997; ). Genetic diversity of rhizobial symbionts isolated from legume species within genera Astragalus, Oxytropis, and Onobrychis. Appl Environ Microbiol 63, 4748–4758.
    [Google Scholar]
  17. Li, J., Yu, L., Li, N. & Wang, H. ( 2000; ). Astragalus mongholicus and Angelica sinensis compound alleviates nephritic hyperlipidemia in rats. Chin Med J 113, 310–314.
    [Google Scholar]
  18. Marmur, J. ( 1961; ). A procedure for the isolation of DNA from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  19. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  20. Martínez-Romero, E., Segovia, L., Mercante, F. M., Franco, A. A., Graham, P. & Pardo, M. A. ( 1991; ). Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int J Syst Bacteriol 41, 417–426.[CrossRef]
    [Google Scholar]
  21. Nagasawa, H., Watanabe, K., Yoshida, M. & Inatomi, H. ( 2001; ). Effects of gold banded lily (Lilium auratum Lindl) or Chinese milk vetch (Astragalus sinicus L) on spontaneous mammary tumourigensis in SHN mice. Anticancer Res 21, 2323–2328.
    [Google Scholar]
  22. Page, R. D. ( 1996; ). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358.
    [Google Scholar]
  23. Peng, G. X., Tan, Z. Y., Wang, E. T., Reinhold-Hurek, B., Chen, W. F. & Chen, W. X. ( 2002; ). Identification of isolates from soybean nodules in Xinjiang Region as Sinorhizobium xinjiangense and genetic differentiation of S. xinjiangense from Sinorhizobium fredii. Int J Syst Evol Microbiol 52, 457–462.
    [Google Scholar]
  24. Shon, Y. H., Kim, J. H. & Nam, K. S. ( 2002; ). Effects of Astragali radix extract on lipopolysaccharide-induced inflammation in human amnion. Biol Pharm Bull 25, 77–80.[CrossRef]
    [Google Scholar]
  25. Sneath, P. H. A. & Sokal, R. R. ( 1973; ). Numerical Taxonomy. The Principles and Practices of Numerical Classification. San Francisco: W. H. Freeman.
  26. Sriprang, R., Hayashi, M., Yamashita, M., Ono, H., Saeki, K. & Murooka, Y. ( 2002; ). A novel bioremediation system for heavy metals using the symbiosis between leguminous plant and genetically engineered rhizobia. J Biotechnol 99, 279–293.[CrossRef]
    [Google Scholar]
  27. Sullivan, J. T., Eardly, B. D., van Berkum, P. & Ronson, C. W. ( 1996; ). Four unnamed species of nonsymbiotic rhizobia isolated from the rhizosphere of Lotus corniculatus. Appl Environ Microbiol 62, 2818–2825.
    [Google Scholar]
  28. Tan, Z. Y., Wang, E. T., Peng, G. X., Zhu, M. E., Martínez-Romero, E. & Chen, W. X. ( 1999; ). Characterization of bacteria isolated from wild legumes in the north-western regions of China. Int J Syst Bacteriol 49, 1457–1469.[CrossRef]
    [Google Scholar]
  29. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  30. Turner, S. L. & Young, J. P. W. ( 2000; ). The glutamine synthetases of rhizobia: phylogenetics and evolutionary implications. Mol Biol Evol 17, 309–319.[CrossRef]
    [Google Scholar]
  31. Velázquez, E., Igual, J. M., Willems, A. & 9 other authors ( 2001; ). Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina). Int J Syst Evol Microbiol 51, 1011–1021.[CrossRef]
    [Google Scholar]
  32. Vincent, J. M. ( 1970; ). A Manual for the Practical Study of Root Nodule Bacteria. IBP Handbook no.15. Oxford: Blackwell Scientific.
  33. Wang, S. Y. & Chen, W. X. ( 1996; ). Numerical taxonomy and DNA relatedness of rhizobia isolated from Astragalus spp. In Diversity and Taxonomy of Rhizobia, pp. 79–84. Edited by F. D. Li et al. Beijing: China Agricultural Scientech Press.
  34. Wang, E. T., van Berkum, P., Sui, X. H., Beyene, D., Chen, W. X. & Martínez-Romero, E. ( 1999; ). Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int J Syst Bacteriol 49, 51–65.[CrossRef]
    [Google Scholar]
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  36. Wdowiak, S. & Malek, W. ( 2000; ). Numerical analysis of Astragalus cicer microsymbionts. Curr Microbiol 41, 142–148.[CrossRef]
    [Google Scholar]
  37. Xu, L. M., Ge, C., Cui, Z., Li, J. & Fan, H. ( 1995; ). Bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans. Int J Syst Bacteriol 45, 706–711.[CrossRef]
    [Google Scholar]
  38. Zhang, X. X., Turner, S. L., Gao, X. W., Yang, H. J., Debelle, F., Yang, G. P., Denarie, J., Young, J. P. W. & Li, F. D. ( 2000; ). The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity. Appl Environ Microbiol 66, 2988–2995.[CrossRef]
    [Google Scholar]
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