1887

Abstract

Ninety-five rhizobial strains isolated from growing in the northern regions of China were classified into three main groups, candidate species I, II and III, based on a polyphasic approach. Comparative analysis of full-length 16S rRNA gene sequences of representative strains showed that candidate species I and II were , while candidate species III, which consisted of non-nodulating strains, was closely related to . The phylogenetic relationships of the three candidate species and some related strains were also confirmed by the sequencing of genes, which were used as an alternative chromosomal marker. The DNA–DNA relatedness was between 11·3 and 47·1 % among representative strains of candidate species I and II and the type strains of defined species. Candidate III had DNA relatedness of between 4·3 and 25·2 % with type strains of and . Two novel species are proposed to accommodate candidate species I and II, sp. nov. (type strain, SDW014=CCBAU 11014=HAMBI 2582) and sp. nov. (type strain, SDW018=CCBAU 11018=HAMBI 2583), respectively. At least two distinct sequences were identified among the strains. The numerically dominant sequence type was most similar to that from the type strain and was identified in strains belonging to the two novel species as well as other, as yet, undefined genome types. Host range studies indicate that the different sequences correlate with different host ranges. Further comparative studies with the defined species are needed to clarify the taxonomic identity of candidate species III.

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2004-11-01
2024-12-03
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