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Volume 61, Issue 11

sp. nov., isolated from a culture of the diatom , and emended description of the genus Free

Abstract

Two Gram-negative-staining, aerobic bacterial strains, designated CL-SC21 and CL-SC22, were isolated from a culture of the diatom (Korean Marine Microalgae Culture Center, KMMCC strain B-396) established from the East Sea, Korea. The two novel strains shared 99.9 % 16S rRNA gene sequence similarity. Analysis of the 16S rRNA gene sequences showed an affiliation with the genus , with the strains sharing 96.5–97.5 % similarity with the type strains of recognized species of the genus and being most closely related to NL21. Phylogenetic analyses of the 16S rRNA gene sequences showed that strain CL-SC21 together with strain CL-SC22 belonged to the genus and formed a robust clade among closely related species. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unidentified aminophospholipids, an unidentified aminolipid, an unidentified phospholipid and five unidentified lipids. Ubiquinone 10 was the major quinone. The major cellular fatty acids of strains CL-SC21 and CL-SC22 were Cω7 (70.6–72.3 %) and Cω8 cyclo (10.9–11.8 %). The genomic DNA G+C contents of strains CL-SC21 and CL-SC22 were 56.7 and 57.1 mol%, respectively. The level of DNA–DNA relatedness between strains CL-SC21 and CL-SC22 was 86 % (reciprocal 91 %), indicating that the two isolates represented a single species. However, levels of DNA–DNA relatedness between NL21 and strains CL-SC21 and CL-SC22 were 28 % (reciprocal 45 %) and 25 % (reciprocal 50 %), respectively. Phylogenetic analysis and the results of biochemical tests showed that strains CL-SC21 and CL-SC22 were different from all recognized species of the genus . Thus, strains CL-SC21 and CL-SC22 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CL-SC21 ( = KCCM 90090 = JCM 17288).

  • Published Online:
Funding
This study was supported by the:
  • Korean Government
  • Ministry of Land, Transport, and Maritime Affairs (MLTM), Korea
  • Ministry of Maritime Affairs and Fisheries (the Korea EAST-1 program)
© 2011 IUMS
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2011-11-01
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Nitratireductor aquimarinus sp. nov., isolated from a culture of the diatom Skeletonema costatum, and emended description of the genus Nitratireductor
Int J Syst Evol Microbiol 61, 2676 (2011); https://doi.org/10.1099/ijs.0.028373-0
/content/journal/ijsem/10.1099/ijs.0.028373-0
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