1887

Abstract

Thirteen Gram-positive-staining coagulase-variable staphylococci were isolated from subclinical and mild clinical mastitic bovine milk ( = 12) and a teat apex ( = 1). The results of sequence analysis of the 16S rRNA gene and two housekeeping genes, and , and DNA fingerprinting with amplified fragment length polymorphism (AFLP) analysis showed that the isolates formed a separate branch within the genus . The phylogenetically most closely related species were and . DNA–DNA hybridization with DSM 20459 and DSM 20674 confirmed that the isolates belonged to a separate species. The predominant fatty acids were i-C, ai-C, i-C and C and the peptidoglycan type was A3α -Lys–Gly. Based on the results of genotypic and phenotypic analyses, it is proposed that the thirteen isolates represent a novel species, for which the name sp. nov. is proposed. Strain 6-4 ( = DSM 23656 = CCUG 59809) is the type strain.

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2012-01-01
2019-11-22
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